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Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea
Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea. In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effac...
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| Published in: | Journal of veterinary diagnostic investigation 2004-11, Vol.16 (6), p.576-578 |
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| Main Authors: | , , , , , , , , |
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| cited_by | cdi_FETCH-LOGICAL-c438t-df68b854ed21486a0a213e4c35a6b753332b547c585c4d5c515646d6034617ab3 |
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| cites | cdi_FETCH-LOGICAL-c438t-df68b854ed21486a0a213e4c35a6b753332b547c585c4d5c515646d6034617ab3 |
| container_end_page | 578 |
| container_issue | 6 |
| container_start_page | 576 |
| container_title | Journal of veterinary diagnostic investigation |
| container_volume | 16 |
| creator | Ha, S.K Hong, K Choi, C.S Jung, K Ha, Y.C Kim, J.H Kim, S.H Yoon, B.S Chae, C.H |
| description | Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea.
In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study. |
| doi_str_mv | 10.1177/104063870401600614 |
| format | article |
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In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study.</description><identifier>ISSN: 1040-6387</identifier><identifier>EISSN: 1943-4936</identifier><identifier>DOI: 10.1177/104063870401600614</identifier><identifier>PMID: 15586575</identifier><language>eng</language><publisher>Los Angeles, CA: J Vet Diagn Invest</publisher><subject>Adhesins, Bacterial - genetics ; Animals ; diarrhea ; Diarrhea - microbiology ; Diarrhea - veterinary ; DNA Primers - genetics ; enterovirulent Escherichia coli group ; Escherichia coli - isolation & purification ; Escherichia coli Infections - microbiology ; Escherichia coli Infections - veterinary ; Escherichia coli Proteins - genetics ; Escherichia infections ; genes ; genetic variation ; Genotype ; piglets ; polymerase chain reaction ; Polymerase Chain Reaction - veterinary ; strain differences ; Swine ; Swine Diseases - microbiology ; weaning</subject><ispartof>Journal of veterinary diagnostic investigation, 2004-11, Vol.16 (6), p.576-578</ispartof><rights>2004 American Association of Veterinary Laboratory Diagnosticians</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-df68b854ed21486a0a213e4c35a6b753332b547c585c4d5c515646d6034617ab3</citedby><cites>FETCH-LOGICAL-c438t-df68b854ed21486a0a213e4c35a6b753332b547c585c4d5c515646d6034617ab3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925,79364</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15586575$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ha, S.K</creatorcontrib><creatorcontrib>Hong, K</creatorcontrib><creatorcontrib>Choi, C.S</creatorcontrib><creatorcontrib>Jung, K</creatorcontrib><creatorcontrib>Ha, Y.C</creatorcontrib><creatorcontrib>Kim, J.H</creatorcontrib><creatorcontrib>Kim, S.H</creatorcontrib><creatorcontrib>Yoon, B.S</creatorcontrib><creatorcontrib>Chae, C.H</creatorcontrib><title>Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea</title><title>Journal of veterinary diagnostic investigation</title><addtitle>J Vet Diagn Invest</addtitle><description>Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea.
In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study.</description><subject>Adhesins, Bacterial - genetics</subject><subject>Animals</subject><subject>diarrhea</subject><subject>Diarrhea - microbiology</subject><subject>Diarrhea - veterinary</subject><subject>DNA Primers - genetics</subject><subject>enterovirulent Escherichia coli group</subject><subject>Escherichia coli - isolation & purification</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Infections - veterinary</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia infections</subject><subject>genes</subject><subject>genetic variation</subject><subject>Genotype</subject><subject>piglets</subject><subject>polymerase chain reaction</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>strain differences</subject><subject>Swine</subject><subject>Swine Diseases - microbiology</subject><subject>weaning</subject><issn>1040-6387</issn><issn>1943-4936</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><recordid>eNp9kU2P1DAMhisEYj_gD3CAnLiVTZqvzhGtFhZpJZBgz5Gbum1GbVPiltX8Bv40Gc1IHJA42Zaf97VlF8UbwT8IYe2N4IobWdschOHcCPWsuBQ7JUu1k-Z5znOnPBIXxRXRnnNdaSteFhdC69poqy-L39_ieJgwASHzA4SZJQS_hjgzmGE8UCAWO4aArMcZGW3NeliQ2JKQcF5ZVsC6gh_C3GdJy7DrwB-LO_IDppA7wHwcAwsUR1ixZV2KE1tCT-wprANrA6Q0ILwqXnQwEr4-x-vi8dPdj9v78uHr5y-3Hx9Kr2S9lm1n6qbWCttKqNoAh0pIVF5qMI3VUsqq0cp6XWuvWu210EaZ1nCpjLDQyOvi_cl3SfHnhrS6KZDHcYQZ40bOWGFFLUwGqxPoUyRK2LklhQnSwQnuji9w_74gi96e3bdmwvav5HzzDNycAIIe3T5uKR-a_m95XngI_fAUEjqaYBzzgMrtf7VBGGectseF353ADqKDPgVyj98rLiTnO6O50vIPwm6mTA</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Ha, S.K</creator><creator>Hong, K</creator><creator>Choi, C.S</creator><creator>Jung, K</creator><creator>Ha, Y.C</creator><creator>Kim, J.H</creator><creator>Kim, S.H</creator><creator>Yoon, B.S</creator><creator>Chae, C.H</creator><general>J Vet Diagn Invest</general><general>SAGE Publications</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea</title><author>Ha, S.K ; Hong, K ; Choi, C.S ; Jung, K ; Ha, Y.C ; Kim, J.H ; Kim, S.H ; Yoon, B.S ; Chae, C.H</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c438t-df68b854ed21486a0a213e4c35a6b753332b547c585c4d5c515646d6034617ab3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adhesins, Bacterial - genetics</topic><topic>Animals</topic><topic>diarrhea</topic><topic>Diarrhea - microbiology</topic><topic>Diarrhea - veterinary</topic><topic>DNA Primers - genetics</topic><topic>enterovirulent Escherichia coli group</topic><topic>Escherichia coli - isolation & purification</topic><topic>Escherichia coli Infections - microbiology</topic><topic>Escherichia coli Infections - veterinary</topic><topic>Escherichia coli Proteins - genetics</topic><topic>Escherichia infections</topic><topic>genes</topic><topic>genetic variation</topic><topic>Genotype</topic><topic>piglets</topic><topic>polymerase chain reaction</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>strain differences</topic><topic>Swine</topic><topic>Swine Diseases - microbiology</topic><topic>weaning</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ha, S.K</creatorcontrib><creatorcontrib>Hong, K</creatorcontrib><creatorcontrib>Choi, C.S</creatorcontrib><creatorcontrib>Jung, K</creatorcontrib><creatorcontrib>Ha, Y.C</creatorcontrib><creatorcontrib>Kim, J.H</creatorcontrib><creatorcontrib>Kim, S.H</creatorcontrib><creatorcontrib>Yoon, B.S</creatorcontrib><creatorcontrib>Chae, C.H</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of veterinary diagnostic investigation</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ha, S.K</au><au>Hong, K</au><au>Choi, C.S</au><au>Jung, K</au><au>Ha, Y.C</au><au>Kim, J.H</au><au>Kim, S.H</au><au>Yoon, B.S</au><au>Chae, C.H</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea</atitle><jtitle>Journal of veterinary diagnostic investigation</jtitle><addtitle>J Vet Diagn Invest</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>16</volume><issue>6</issue><spage>576</spage><epage>578</epage><pages>576-578</pages><issn>1040-6387</issn><eissn>1943-4936</eissn><abstract>Department of Veterinary Pathology, College of Veterinary Medicine and School of Agricultural Biotechnology, Seoul National University, Seoul, Kwanak-Gu 151-742, Republic of Korea.
In this study the subtype of eae gene was determined by polymerase chain reaction for a total of 59 attaching and effacing Escherichia coli isolated from preweaned (38 isolates) and postweaned (21 isolates) pigs. The eae(beta) gene detected in 19 E. coli from preweaned pigs and 10 E. coli from postweaned pigs was found to be the most common subtype, followed by eae(gamma), eae(epsilon), and eae(zeta) genes. Subtypes were not determined for 7 E. coli isolates. No other subtype of the eae gene was detected in eae+ E. coli evaluated in this study.</abstract><cop>Los Angeles, CA</cop><pub>J Vet Diagn Invest</pub><pmid>15586575</pmid><doi>10.1177/104063870401600614</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Journal of veterinary diagnostic investigation, 2004-11, Vol.16 (6), p.576-578 |
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| language | eng |
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| source | Sage Journals Online |
| subjects | Adhesins, Bacterial - genetics Animals diarrhea Diarrhea - microbiology Diarrhea - veterinary DNA Primers - genetics enterovirulent Escherichia coli group Escherichia coli - isolation & purification Escherichia coli Infections - microbiology Escherichia coli Infections - veterinary Escherichia coli Proteins - genetics Escherichia infections genes genetic variation Genotype piglets polymerase chain reaction Polymerase Chain Reaction - veterinary strain differences Swine Swine Diseases - microbiology weaning |
| title | Polymerase chain reaction analysis of eae gene subtypes present in attaching and effacing Escherichia coli isolated from pigs with diarrhea |
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