Characterization and application of the selective strand annealing activity of the N terminal domain of hepatitis delta antigen

We used synthetic DNA oligos to investigate the nucleic acid chaperone properties of the N terminal domain of hepatitis delta antigen (NdAg). We found that NdAg possessed a bona fide chaperone activity. NdAg could distinguish subtle differences in the thermal stability of the base pairing region, an...

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Bibliographic Details
Published in:FEBS letters 2004-12, Vol.578 (3), p.345-350
Main Authors: Huang, Zhi-Shun, Chen, Albert You-Ju, Wu, Huey-Nan
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Pairing
Base Sequence
Escherichia coli - genetics
Hepatitis delta antigen
Hepatitis delta Antigens - chemistry
Hepatitis delta Antigens - genetics
Hepatitis delta Antigens - metabolism
Hepatitis Delta Virus - genetics
Hepatitis Delta Virus - metabolism
Hybridization
Immunoblotting
In Situ Hybridization
Molecular Chaperones - chemistry
Molecular Chaperones - metabolism
Molecular Sequence Data
Nucleic acid chaperone
Protein Structure, Tertiary
Strand annealing
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Summary:We used synthetic DNA oligos to investigate the nucleic acid chaperone properties of the N terminal domain of hepatitis delta antigen (NdAg). We found that NdAg possessed a bona fide chaperone activity. NdAg could distinguish subtle differences in the thermal stability of the base pairing region, and enabled DNA oligos to form a more stable duplex among competing sequences through facilitating strand annealing selectively, stimulating duplex conversion selectively, and stabilizing the more stable duplex. The property of NdAg identified in this study could be applied to improve the efficiency and specificity of dot blot hybridization under conditions of low stringency.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2004.11.043