Stimulation of osteoblastic differentiation and inhibition of interleukin-6 and nitric oxide in MC3T3-E1 cells by pomegranate ethanol extract

In this experiment, we studied the effects of pomegranate fruit extract (PE) on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. PE (16~250 μg/ml) significantly increased the growth of MC3T3-E1 cells (P < 0.05). Moreover, PE (50 μg/ml) caused a signi...

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Bibliographic Details
Published in:Phytotherapy research 2009-05, Vol.23 (5), p.737-739
Main Authors: Kim, Young Ho, Choi, Eun Mi
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - metabolism
Animals
Biological and medical sciences
Cell Differentiation - drug effects
Cell Line
Collagen - metabolism
differentiation
General pharmacology
Interleukin-6 - metabolism
Medical sciences
Mice
Nitric Oxide - metabolism
osteoblastic MC3T3-E1 cells
Osteoblasts - drug effects
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Extracts - pharmacology
pomegranate
Punicaceae - chemistry
Tumor Necrosis Factor-alpha - metabolism
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Summary:In this experiment, we studied the effects of pomegranate fruit extract (PE) on the function of osteoblastic MC3T3-E1 cells and the production of local factors in osteoblasts. PE (16~250 μg/ml) significantly increased the growth of MC3T3-E1 cells (P < 0.05). Moreover, PE (50 μg/ml) caused a significant elevation of alkaline phosphatase (ALP) activity and collagen content in the cells. We then examined the effect of PE on the TNF-α-induced production of interleukin-6 (IL-6) and nitric oxide (NO) in osteoblasts. Treatment with PE (10~50 μg/ml) decreased the TNF-α (10⁻¹⁰ M)-induced production of IL-6 and NO in osteoblasts. Copyright © 2008 John Wiley & Sons, Ltd.
ISSN:0951-418X
1099-1573
DOI:10.1002/ptr.2587