Automation of the linear array HPV genotyping test and its application for routine typing of human papillomaviruses in cervical specimens of women without cytological abnormalities in Switzerland

• Published in: Journal of clinical virology 2009-05, Vol.45 (1), p.23-27
• Main Authors: Dobec, Marinko, Bannwart, Fridolin, Kaeppeli, Franz, Cassinotti, Pascal
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Allergy and Immunology; Automated linear array HPV test; Automation; Biological and medical sciences; Cervix Uteri - cytology; Cervix Uteri - virology; Data Interpretation, Statistical; DNA, Viral - analysis; Epidemiology; Female; Fundamental and applied biological sciences. Psychology; Genotype; HPV molecular epidemiology; HPV typing; Human papillomavirus; Human viral diseases; Humans; Infectious Disease; Infectious diseases; Medical sciences; Microbiology; Middle Aged; Miscellaneous; Molecular Epidemiology; Oligonucleotide Array Sequence Analysis - methods; Papillomaviridae - classification; Papillomaviridae - genetics; Papillomavirus Infections - diagnosis; Papillomavirus Infections - epidemiology; Papillomavirus Infections - virology; Reagent Kits, Diagnostic - virology; Switzerland; Time Factors; Viral diseases; Virology
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2009.03.005

Abstract Background There is a need for reliable, automated high throughput HPV detection and genotyping methods for pre- and post-prophylactic vaccine intervention analyses. Objectives To optimize the linear array (LA) HPV genotyping test (Roche Diagnostics, Rotkreuz) in regard to possible automation steps for the routine laboratory diagnosis of HPV infections and to analyze the HPV genotype distribution in cervical specimens of women without cytological abnormalities in Switzerland. Study design 680 cervical cell specimens with normal cytology, obtained from women undergoing routine cervical screening by liquid-based Pap smear, were analyzed by the LA HPV genotyping test for HPV-DNA. Results The automation of the LA HPV genotyping test resulted in a total hands-on time reduction of 255 min (from 480 to 225 min; 53%). Any of 37 HPV genotypes were detected in 117 (17.2%) and high-risk (HR) HPV in 55 (8.1%) of 680 women with normal cytology. The highest prevalence of any HPV (28.1%) and HR-HPV (15.1%) was observed in age-group 21–30 and showed a continuous decrease in older age-groups. The most common HR-HPV genotypes were HPV-16 (12%), HPV-31 (9.4%), HPV-52 (6%), HPV-51 (5.1%), HPV-45 (4.3%), HPV-58 (4.3%) and HPV-59 (4.3%). Conclusions The optimization and automation of the LA HPV genotyping test makes it suited for high throughput HPV detection and typing. The epidemiological data provides information about distribution of HPV genotypes in women without cytological abnormalities in Switzerland and may be important for determining the future impact of vaccines and potential changes in the country's epidemiological HPV profile.