Evaluation of Pre transplant T-Cell activation status by soluble CD 30 determination

To evaluate the utility of serum CD30 (sCD30) levels as predictor of early acute graft rejection in live related renal transplant programme. This prospective study included 50 consecutive renal transplant recipients who received their first live related renal allograft at the Sindh Institute of Urol...

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Bibliographic Details
Published in:Journal of the Pakistan Medical Association 2009-04, Vol.59 (4), p.212-215
Main Authors: ABBAS, Khawar, MUZAFFAR, Rana, MIRZA NAQI ZAFAR, MUBARAK, Muhammad, SYED ALI ANWAR NAQVI, SYED ADIBUL HASSAN RIZVI
Format: Article
Language:English
Subjects:
Adolescent
Adult
B-Lymphocytes - immunology
Biological and medical sciences
Biomarkers - blood
Case-Control Studies
Child
Child, Preschool
Female
General aspects
Graft Rejection - blood
Graft Rejection - diagnosis
Graft Rejection - immunology
Graft Survival - immunology
Humans
Ki-1 Antigen - blood
Ki-1 Antigen - immunology
Kidney Transplantation - immunology
Lymphocyte Activation - immunology
Male
Medical sciences
Middle Aged
Prospective Studies
T-Lymphocytes - immunology
Tissue Donors
Treatment Outcome
Young Adult
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Summary:To evaluate the utility of serum CD30 (sCD30) levels as predictor of early acute graft rejection in live related renal transplant programme. This prospective study included 50 consecutive renal transplant recipients who received their first live related renal allograft at the Sindh Institute of Urology and Transplantation (SIUT) between October 2006 and March 2007. Blood samples were obtained one day before transplantation and on the third and fourteenth posttransplant days. Blood samples were also obtained from 50, age and sex matched healthy control individuals. Levels of serum sCD30 were measured by Enzyme Linked Immunosorbent Assay (ELISA). Donor-recipient blood group matching was identical in all patients. Pre-transplant lymphocyte crossmatch for T and B cells was negative, and panel reactive antibodies (PRA) were 0% for all recipients. The mean age of recipients was 31.6 +/- 10.23 years (range 5 to 55 years), while mean donor age was 32.74 +/- 8.48 years (range 21-50 years). Eleven (22%) recipients and donors were HLA identical while remaining (78%) were one haplotype match. Average serum sCD30 pre-transplant levels (37.8 +/- 4.97U/ml) were significantly higher than those of healthy individual's mean value of 8.48 +/- 4.97 U/ml, (P = 0.001). Eight (16%) patients developed acute rejection episode during this follow up period. Rejections were described and classified according to BANFF 97 classification. In this small single center study the serum levels of sCD30 did not show any significant difference between rejection and non rejection group in our transplant population.
ISSN:0030-9982