EPR imaging to estimate the in vivo intracerebral reducing ability of mature rats after neonatal hypoxic–ischemic brain injury

A rat model of neonatal hypoxic–ischemic encephalopathy (Rice's model) was obtained by unilateral ligation of the common carotid artery of 7-day-old rats with hypoxia (exposure to 8% oxygen). To estimate the in vivo intracerebral reducing ability of the mature rats (8 weeks old) of Rice's...

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Bibliographic Details
Published in:Magnetic resonance imaging 2004-11, Vol.22 (9), p.1305-1309
Main Authors: Yokoyama, Hidekatsu, Ueda, Yuto, Itoh, Osamu, Ikeda, Tomoaki, Noor, Jesmin I., Ikenoue, Tuyomu
Format: Article
Language:English
Subjects:
Animals
Animals, Newborn
Antipyrine - analogs & derivatives
Antipyrine - therapeutic use
Blood-Brain Barrier - metabolism
Brain - drug effects
Brain - metabolism
Carotid Artery, Common - physiopathology
Cyclic N-Oxides - pharmacokinetics
Disease Models, Animal
Electron Spin Resonance Spectroscopy - methods
Free Radical Scavengers - therapeutic use
Hypoxia-Ischemia, Brain - etiology
Hypoxia-Ischemia, Brain - metabolism
In vivo EPR imaging
Ligation - methods
Neonatal brain hypoxic–ischemic injury
Neuroprotective Agents - pharmacokinetics
Oxidation-Reduction
Pyrrolidines
Rats
Rats, Wistar
Reducing ability
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Summary:A rat model of neonatal hypoxic–ischemic encephalopathy (Rice's model) was obtained by unilateral ligation of the common carotid artery of 7-day-old rats with hypoxia (exposure to 8% oxygen). To estimate the in vivo intracerebral reducing ability of the mature rats (8 weeks old) of Rice's model, temporal electron paramagnetic resonance (EPR) imaging of the brain of a rat receiving a blood–brain barrier-permeable nitroxide radical, 3-hydroxymethyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl, was performed. In this imaging technique, the decay rate of the EPR signal intensity in a selected region of the brain is indicative of region-specific reducing ability. The effect of neonatal treatment of an antioxidant agent, 3-methyl-1-phenyl-2-pyrazolin-5-one (MCI-186), after a hypoxic–ischemic insult was also tested. It was found that the reducing ability had been depleted in the contralateral hemisphere of Rice's model rats; this depletion was suppressed by administering MCI-186.
ISSN:0730-725X
1873-5894
DOI:10.1016/j.mri.2004.09.003