Temperature and pH-stability of commercial stationary phases

In this paper, the temperature and pH stability of silica-based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 1...

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Bibliographic Details
Published in:Journal of separation science 2009-05, Vol.32 (9), p.1262-1274
Main Authors: Teutenberg, Thorsten, Hollebekkers, Koen, Wiese, Steffen, Boergers, Andrea
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
High temperature LC
Other chromatographic methods
Stationary phase
Temperature stability
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Summary:In this paper, the temperature and pH stability of silica-based RP stationary phases were investigated. Furthermore, nonsiliceous phases like a polymeric column based on polystyrene divinylbenzene and a polybutadiene coated zirconium dioxide column were also included. The columns were heated up to 150°C at dynamic conditions, which means that the eluent consisting of water and methanol (90:10, v/v) was continuously purged through the packed bed. After every 5 h, the columns were cooled down to room temperature and the efficiency was measured by injecting a test sample based on the Neue test. It could be shown that some stationary phases exhibited a very good temperature stability at the test conditions specified above.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.200800712