Proteome mapping of the Drosophila melanogaster male reproductive system

The fruit fly Drosophila melanogaster is an excellent model organism for studying insect reproductive biology. Although the gene expression profiles of both male and female reproductive organs have been studied in detail, their proteomic profiles and functional characteristics largely remained to be...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2009-05, Vol.9 (9), p.2484-2493
Main Authors: Takemori, Nobuaki, Yamamoto, Masa-Toshi
Format: Article
Language:English
Subjects:
2‐D proteome reference map
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Drosophila melanogaster
Drosophila melanogaster - physiology
Electrophoresis, Gel, Two-Dimensional
Fundamental and applied biological sciences. Psychology
Genitalia, Male - anatomy & histology
Genitalia, Male - chemistry
Genitalia, Male - metabolism
Insect Proteins - analysis
Insect Proteins - metabolism
Insect reproduction
MALDI‐QIT‐TOF MS
MALDI‐TOF MS
Male
Miscellaneous
Models, Biological
Peptide Mapping
Proteins
Proteomics
Seminal fluid
Seminal Plasma Proteins - analysis
Seminal Plasma Proteins - metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Sperm
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Summary:The fruit fly Drosophila melanogaster is an excellent model organism for studying insect reproductive biology. Although the gene expression profiles of both male and female reproductive organs have been studied in detail, their proteomic profiles and functional characteristics largely remained to be clarified. In this study, we conducted proteome mapping of the male internal reproductive organs using 2-DE. We identified a total of 440 protein components from gels of the male reproductive organs (testis, seminal vesicle, accessory gland, ejaculatory duct, and ejaculatory bulb). A number of proteins associated with odorant/pheromone-binding, lipid metabolism, proteolysis, and antioxidation were expressed tissue specifically in the male reproductive system. Based on our proteomic data set, we constructed reference proteome maps of the reproductive organs, which will provide valuable information toward a comprehensive understanding of Drosophila reproduction.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200800795