Development of a sensitive and selective LC/MS/MS method for the simultaneous determination of intracellular 1-beta-d-arabinofuranosylcytosine triphosphate (araCTP), cytidine triphosphate (CTP) and deoxycytidine triphosphate (dCTP) in a human follicular lymphoma cell line

A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, a...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2009-05, Vol.877 (14-15), p.1417-1425
Main Authors: Crauste, Céline, Lefebvre, Isabelle, Hovaneissian, Michael, Puy, Jean Yves, Roy, Béatrice, Peyrottes, Suzanne, Cohen, Sabine, Guitton, Jérôme, Dumontet, Charles, Perigaud, Christian
Format: Article
Language:English
Subjects:
Analysis
Analytical, structural and metabolic biochemistry
Arabinofuranosylcytosine Triphosphate - chemistry
araCTP
Biological and medical sciences
Cell Line, Tumor
Chemical Sciences
Chromatography, High Pressure Liquid - methods
CTP
Cytidine Triphosphate - chemistry
Deoxycytosine Nucleotides - chemistry
Fundamental and applied biological sciences. Psychology
General pharmacology
Humans
LC/MS/MS
Lymphoma, Follicular - chemistry
Medical sciences
Organic chemistry
Pharmacology. Drug treatments
Porous graphitic carbon
Sensitivity and Specificity
Tandem Mass Spectrometry - methods
Thionucleotides - chemistry
WAX
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Summary:A method was developed for the quantification of araCTP, CTP and dCTP in a human follicular lymphoma cell line. This method involves solid phase extraction (SPE) using a weak anion-exchanger (WAX) cartridge, a porous graphitic carbon high-performance liquid chromatography (HPLC) column separation, and tandem mass spectrometry (MS/MS) detection. By using a triple quadrupole mass spectrometer operating in negative ion multiple reaction monitoring (MRM) mode, the method was able to achieve a lower limit of quantification (LLOQ) of 0.1μgmL−1 for araCTP and of 0.01μgmL−1 for both CTP and dCTP. The method was validated and used to determine the amount of araCTP, CTP and dCTP formed after incubation of araC and an araCMP prodrug in the human follicular lymphoma cell line RL.
ISSN:1570-0232
0378-4347
1873-376X
DOI:10.1016/j.jchromb.2009.02.071