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Intracellular Localization of CD14 Protein in Intestinal Macrophages
Background: Our research is focused on intestinal macrophages, which are believed to contribute to the maintenance of intestinal homeostasis. In addition, intestinal macrophages are unique in that there is an impairment of expression of tumor necrosis factor (TNF) from lipopolysaccharide (LPS). This...
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| Published in: | Anticancer research 2009-03, Vol.29 (3), p.865-869 |
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| container_title | Anticancer research |
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| creator | YOSHIOKA, Noriko TANIGUCHI, Yoshie YOSHIDA, Aya NAKATA, Kazue NISHIZAWA, Takashi INAGAWA, Hiroyuki KOHCHI, Chie SOMA, Gen-Ichiro |
| description | Background: Our research is focused on intestinal macrophages, which are believed to contribute to the maintenance of intestinal
homeostasis. In addition, intestinal macrophages are unique in that there is an impairment of expression of tumor necrosis
factor (TNF) from lipopolysaccharide (LPS). This characteristic can be attributed to the lack or poor level of expression
of toll-like receptor 4 (TLR4) or CD14 on the membrane of intestinal macrophages. We therefore decided to identify where CD14
was localized in intestinal macrophages. Materials and Methods: The endoplasmic reticulum and Golgi apparatus were double
stained and the intracellular localization in the intestinal macrophages was observed using a confocal laser microscope. Results:
CD14 of peritoneal macrophages was expressed both in the endoplasmic reticulum and Golgi apparatus. By contrast, intestinal
macrophages expressed very little CD14 on the cellular membrane. CD14 was present in the endoplasmic reticulum of intestinal
macrophages, but was rare in the Golgi apparatus. Conclusion: The lack of expression of CD14 on the cell membrane of intestinal
macrophages may be caused by transport interference from the endoplasmic reticulum to the Golgi apparatus. |
| format | article |
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homeostasis. In addition, intestinal macrophages are unique in that there is an impairment of expression of tumor necrosis
factor (TNF) from lipopolysaccharide (LPS). This characteristic can be attributed to the lack or poor level of expression
of toll-like receptor 4 (TLR4) or CD14 on the membrane of intestinal macrophages. We therefore decided to identify where CD14
was localized in intestinal macrophages. Materials and Methods: The endoplasmic reticulum and Golgi apparatus were double
stained and the intracellular localization in the intestinal macrophages was observed using a confocal laser microscope. Results:
CD14 of peritoneal macrophages was expressed both in the endoplasmic reticulum and Golgi apparatus. By contrast, intestinal
macrophages expressed very little CD14 on the cellular membrane. CD14 was present in the endoplasmic reticulum of intestinal
macrophages, but was rare in the Golgi apparatus. Conclusion: The lack of expression of CD14 on the cell membrane of intestinal
macrophages may be caused by transport interference from the endoplasmic reticulum to the Golgi apparatus.</description><identifier>ISSN: 0250-7005</identifier><identifier>EISSN: 1791-7530</identifier><identifier>PMID: 19414321</identifier><language>eng</language><publisher>Attiki: International Institute of Anticancer Research</publisher><subject>Animals ; Biological and medical sciences ; Endoplasmic Reticulum - metabolism ; Golgi Apparatus - metabolism ; Immunoenzyme Techniques ; Intestines - cytology ; Lipopolysaccharide Receptors - metabolism ; Macrophages - metabolism ; Male ; Medical sciences ; Mice ; Mice, Inbred C3H ; Tumors</subject><ispartof>Anticancer research, 2009-03, Vol.29 (3), p.865-869</ispartof><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>309,310,314,780,784,789,790,23930,23931,25140</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21515397$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19414321$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOSHIOKA, Noriko</creatorcontrib><creatorcontrib>TANIGUCHI, Yoshie</creatorcontrib><creatorcontrib>YOSHIDA, Aya</creatorcontrib><creatorcontrib>NAKATA, Kazue</creatorcontrib><creatorcontrib>NISHIZAWA, Takashi</creatorcontrib><creatorcontrib>INAGAWA, Hiroyuki</creatorcontrib><creatorcontrib>KOHCHI, Chie</creatorcontrib><creatorcontrib>SOMA, Gen-Ichiro</creatorcontrib><title>Intracellular Localization of CD14 Protein in Intestinal Macrophages</title><title>Anticancer research</title><addtitle>Anticancer Res</addtitle><description>Background: Our research is focused on intestinal macrophages, which are believed to contribute to the maintenance of intestinal
homeostasis. In addition, intestinal macrophages are unique in that there is an impairment of expression of tumor necrosis
factor (TNF) from lipopolysaccharide (LPS). This characteristic can be attributed to the lack or poor level of expression
of toll-like receptor 4 (TLR4) or CD14 on the membrane of intestinal macrophages. We therefore decided to identify where CD14
was localized in intestinal macrophages. Materials and Methods: The endoplasmic reticulum and Golgi apparatus were double
stained and the intracellular localization in the intestinal macrophages was observed using a confocal laser microscope. Results:
CD14 of peritoneal macrophages was expressed both in the endoplasmic reticulum and Golgi apparatus. By contrast, intestinal
macrophages expressed very little CD14 on the cellular membrane. CD14 was present in the endoplasmic reticulum of intestinal
macrophages, but was rare in the Golgi apparatus. Conclusion: The lack of expression of CD14 on the cell membrane of intestinal
macrophages may be caused by transport interference from the endoplasmic reticulum to the Golgi apparatus.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Endoplasmic Reticulum - metabolism</subject><subject>Golgi Apparatus - metabolism</subject><subject>Immunoenzyme Techniques</subject><subject>Intestines - cytology</subject><subject>Lipopolysaccharide Receptors - metabolism</subject><subject>Macrophages - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Tumors</subject><issn>0250-7005</issn><issn>1791-7530</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqF0M9LwzAUwPEgipvTf0F6UG-F_Gia5iibPwYTPeg5vGbJGknbmbSI_vVmbHoVHrzLh8eXd4SmREiSC87wMZpiynEuMOYTdBbjO8ZlKSt2iiZEFqRglEzRYtkNAbTxfvQQslWvwbtvGFzfZb3N5gtSZC-hH4zrsjRJmzi4Dnz2BDr02wY2Jp6jEws-movDnqG3-7vX-WO-en5Yzm9XeUOlHHKoATSlgpSVqGrDRV3Z0hjDwBJWUcJxBbw0GuxapjyLeVFYDEJoKTWXwGboZn93G_qPMYWo1sVdO3SmH6MqBcVYFsW_kGIiU8UOXh7gWLdmrbbBtRC-1O-DErg-AIjpNTZAp138cymacCZFcld717hN8-mCUbEF79NZpiBQqZiqSs5-AATxe0E</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>YOSHIOKA, Noriko</creator><creator>TANIGUCHI, Yoshie</creator><creator>YOSHIDA, Aya</creator><creator>NAKATA, Kazue</creator><creator>NISHIZAWA, Takashi</creator><creator>INAGAWA, Hiroyuki</creator><creator>KOHCHI, Chie</creator><creator>SOMA, Gen-Ichiro</creator><general>International Institute of Anticancer Research</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>Intracellular Localization of CD14 Protein in Intestinal Macrophages</title><author>YOSHIOKA, Noriko ; TANIGUCHI, Yoshie ; YOSHIDA, Aya ; NAKATA, Kazue ; NISHIZAWA, Takashi ; INAGAWA, Hiroyuki ; KOHCHI, Chie ; SOMA, Gen-Ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h299t-abaac22716878be57b8f6eee3af13821508a56ecafd9143f0544f0a77c99c59a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Endoplasmic Reticulum - metabolism</topic><topic>Golgi Apparatus - metabolism</topic><topic>Immunoenzyme Techniques</topic><topic>Intestines - cytology</topic><topic>Lipopolysaccharide Receptors - metabolism</topic><topic>Macrophages - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOSHIOKA, Noriko</creatorcontrib><creatorcontrib>TANIGUCHI, Yoshie</creatorcontrib><creatorcontrib>YOSHIDA, Aya</creatorcontrib><creatorcontrib>NAKATA, Kazue</creatorcontrib><creatorcontrib>NISHIZAWA, Takashi</creatorcontrib><creatorcontrib>INAGAWA, Hiroyuki</creatorcontrib><creatorcontrib>KOHCHI, Chie</creatorcontrib><creatorcontrib>SOMA, Gen-Ichiro</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Anticancer research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>YOSHIOKA, Noriko</au><au>TANIGUCHI, Yoshie</au><au>YOSHIDA, Aya</au><au>NAKATA, Kazue</au><au>NISHIZAWA, Takashi</au><au>INAGAWA, Hiroyuki</au><au>KOHCHI, Chie</au><au>SOMA, Gen-Ichiro</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intracellular Localization of CD14 Protein in Intestinal Macrophages</atitle><jtitle>Anticancer research</jtitle><addtitle>Anticancer Res</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>29</volume><issue>3</issue><spage>865</spage><epage>869</epage><pages>865-869</pages><issn>0250-7005</issn><eissn>1791-7530</eissn><abstract>Background: Our research is focused on intestinal macrophages, which are believed to contribute to the maintenance of intestinal
homeostasis. In addition, intestinal macrophages are unique in that there is an impairment of expression of tumor necrosis
factor (TNF) from lipopolysaccharide (LPS). This characteristic can be attributed to the lack or poor level of expression
of toll-like receptor 4 (TLR4) or CD14 on the membrane of intestinal macrophages. We therefore decided to identify where CD14
was localized in intestinal macrophages. Materials and Methods: The endoplasmic reticulum and Golgi apparatus were double
stained and the intracellular localization in the intestinal macrophages was observed using a confocal laser microscope. Results:
CD14 of peritoneal macrophages was expressed both in the endoplasmic reticulum and Golgi apparatus. By contrast, intestinal
macrophages expressed very little CD14 on the cellular membrane. CD14 was present in the endoplasmic reticulum of intestinal
macrophages, but was rare in the Golgi apparatus. Conclusion: The lack of expression of CD14 on the cell membrane of intestinal
macrophages may be caused by transport interference from the endoplasmic reticulum to the Golgi apparatus.</abstract><cop>Attiki</cop><pub>International Institute of Anticancer Research</pub><pmid>19414321</pmid><tpages>5</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Endoplasmic Reticulum - metabolism Golgi Apparatus - metabolism Immunoenzyme Techniques Intestines - cytology Lipopolysaccharide Receptors - metabolism Macrophages - metabolism Male Medical sciences Mice Mice, Inbred C3H Tumors |
| title | Intracellular Localization of CD14 Protein in Intestinal Macrophages |
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