Hypoxia Is Important in the Biology and Aggression of Human Glial Brain Tumors

• Published in: Clinical cancer research 2004-12, Vol.10 (24), p.8177-8184
• Main Authors: EVANS, Sydney M, JUDY, Kevin D, COLLINS, Ruth A, GRADY, M. Sean, KOCH, Cameron J, DUNPHY, Isolde, JENKINS, W. Timothy, HWANG, Wei-Ting, NELSON, Peter T, LUSTIG, Robert A, JENKINS, Kevin, MAGARELLI, Deirdre P, HAHN, Stephen M
• Format: Article
• Language: English
• Subjects: Aged; Antineoplastic agents; Biological and medical sciences; Brain Neoplasms - diagnostic imaging; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Electrodes; Etanidazole - analogs & derivatives; Etanidazole - metabolism; Glioma - diagnostic imaging; Glioma - metabolism; Glioma - pathology; Humans; Hydrocarbons, Fluorinated - metabolism; Hypoxia - metabolism; Hypoxia - pathology; Indicators and Reagents; Medical sciences; Middle Aged; Needles; Neoplasm Recurrence, Local - diagnostic imaging; Neoplasm Recurrence, Local - metabolism; Neoplasm Recurrence, Local - pathology; Pharmacology. Drug treatments; Radiography; Time Factors
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.CCR-04-1081

We investigated whether increasing levels of tissue hypoxia, measured by the binding of EF5 [2-(2-nitro-1- H -imidazol-1-yl)- N -(2,2,3,3,3-pentafluoropropyl) acetamide] or by Eppendorf needle electrodes, were associated with tumor aggressiveness in patients with previously untreated glial brain tumors. We hypothesized that more extensive and severe hypoxia would be present in tumor cells from patients bearing more clinically aggressive tumors. Hypoxia was measured with the 2-nitroimidazole imaging agent EF5 in 18 patients with supratentorial glial neoplasms. In 12 patients, needle electrode measurements were made intraoperatively. Time to recurrence was used as an indicator of tumor aggression and was analyzed as a function of EF5 binding, electrode values and recursive partitioning analysis (RPA) classification. On the basis of EF5 binding, WHO grade 2 tumors were characterized by modest cellular hypoxia (pO 2 s ≈ 10%) and grade 3 tumors by modest-to-moderate hypoxia (pO 2 s ≈ 10%- 2.5%). Severe hypoxia (≈0.1% oxygen) was present in 5 of 12 grade 4 tumors. A correlation between more rapid tumor recurrence and hypoxia was demonstrated with EF5 binding, but this relationship was not predicted by Eppendorf measurements.