Phylogenetic Relationships of Rhizobia Based on Citrate Synthase Gene Sequences

Partial nucleotide sequences of the citrate synthase ( gltA) gene from different rhizobia genera were determined. Tree topologies based on this housekeeping gene were similar to that obtained using 16S rRNA sequences. However gltA appeared to be more reliable at determining phylogenetic relationship...

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Bibliographic Details
Published in:Systematic and applied microbiology 2004-11, Vol.27 (6), p.703-706
Main Authors: Hernández-Lucas, Ismael, Rogel-Hernández, Marco Antonio, Segovia, Lorenzo, Rojas-Jiménez, Keilor, Martínez-Romero, Esperanza
Format: Article
Language:English
Subjects:
16S rRNA
Alphaproteobacteria - classification
Alphaproteobacteria - enzymology
Alphaproteobacteria - genetics
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Citrate (si)-Synthase - genetics
DNA, Bacterial - chemistry
DNA, Bacterial - isolation & purification
DNA, Ribosomal - chemistry
DNA, Ribosomal - isolation & purification
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes, rRNA
gltA
Microbiology
Molecular Sequence Data
Phylogeny
Rhizobiaceae - classification
Rhizobiaceae - enzymology
Rhizobiaceae - genetics
Rhizobium
RNA, Bacterial - genetics
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
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Summary:Partial nucleotide sequences of the citrate synthase ( gltA) gene from different rhizobia genera were determined. Tree topologies based on this housekeeping gene were similar to that obtained using 16S rRNA sequences. However gltA appeared to be more reliable at determining phylogenetic relationships of closely related taxa. We propose gltA sequences as an additional tool to be used in molecular phylogenetic studies.
ISSN:0723-2020
1618-0984
DOI:10.1078/0723202042369893