Quenched emission of fluorescence by ligand functionalized gold nanoparticles

A fluorescence-based detection scheme that uses ligand functionalized gold nanoparticles is proposed. The transduction scheme is based on the strong quenching of the fluorescence emission exerted by metallic surfaces on fluorophores positioned in their immediate vicinity (< 5 nm). Binding of fluo...

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Bibliographic Details
Published in:Journal of fluorescence 2004-07, Vol.14 (4), p.401-405
Main Authors: Aslan, Kadir, Pérez-Luna, Víctor H
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - chemistry
Antibodies, Monoclonal - immunology
Binding, Competitive
Biosensing Techniques - methods
Biotin - chemistry
Biotin - immunology
Biotinylation - methods
Cross-Linking Reagents - chemistry
Fluorescence
Fluorescent Dyes - chemistry
Gold - chemistry
Ligands
Molecular Structure
Nanostructures - chemistry
Nanotechnology - methods
Spectrometry, Fluorescence
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Summary:A fluorescence-based detection scheme that uses ligand functionalized gold nanoparticles is proposed. The transduction scheme is based on the strong quenching of the fluorescence emission exerted by metallic surfaces on fluorophores positioned in their immediate vicinity (< 5 nm). Binding of fluorophore-labeled anti-biotin to biotinylated gold nanoparticles resulted in decreased fluorescence emission intensity. Subsequent competitive dissociation of labeled anti-biotin with D-biotin resulted in increased fluorescence emission intensity. These interactions occurred by means of specific molecular recognition because when the binding sites of anti-biotin were saturated with D-biotin prior to interaction with the gold nanoparticles; changes in the fluorescence emission intensity were not observed.
ISSN:1053-0509
DOI:10.1023/B:JOFL.0000031821.74706.ea