Adiponectin Receptor-1 Expression Is Decreased in the Pancreas of Obese Mice

Background Obesity is epidemic in the 21st century and has been shown to be a risk factor for developing severe acute pancreatitis. Adipose tissue produces small molecules called adipokines, which are important in modulating metabolism and inflammation. The anti-inflammatory adipokine adiponectin is...

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Bibliographic Details
Published in:The Journal of surgical research 2009-06, Vol.154 (1), p.78-84
Main Authors: Wade, Terence E., M.D, Mathur, Abhishek, M.D, Lu, Debao, M.D, Swartz-Basile, Deborah A., Ph.D, Pitt, Henry A., M.D, Zyromski, Nicholas J., M.D
Format: Article
Language:English
Subjects:
adiponectin
adiponectin receptors
Animals
Biological and medical sciences
Body Weight
DNA Primers
Female
General aspects
Immunohistochemistry
Leptin - deficiency
Leptin - genetics
Medical sciences
Metabolic diseases
Mice
Mice, Inbred C57BL
Mice, Obese
Obesity
Obesity - genetics
Pancreas - physiopathology
pancreatitis
Receptors, Adiponectin - genetics
Reverse Transcriptase Polymerase Chain Reaction
Surgery
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Summary:Background Obesity is epidemic in the 21st century and has been shown to be a risk factor for developing severe acute pancreatitis. Adipose tissue produces small molecules called adipokines, which are important in modulating metabolism and inflammation. The anti-inflammatory adipokine adiponectin is decreased in obesity and inversely mirrors the severity of pancreatitis in a murine experimental model. Adiponectin acts through two receptors, AdipoR1 and AdipoR2; no data are currently available regarding adiponection receptor expression in the obese murine pancreas. Materials and methods Immunohistochemical and reverse transcription-polymerase chain reaction analysis were undertaken to determine expression of adiponectin receptors AdipoR1 and AdipoR2 in the pancreas and liver of lean (C57BL/6J) and congenitally obese (LepOb and LepDb ) mice. Results Immunohistochemistry confirmed expression of both AdipoR1 and AdipoR2 in the pancreas of all three murine strains. Staining was positive in acinar cells and to a lesser extent in islet cells. Pancreatic gene expression of AdipoR2 was similar among lean and obese mice. AdipoR1 gene expression, however, was significantly ( P < 0.001) decreased in the pancreas of both LepOb and LepDb mice compared to wild-type lean animals. Gene expression of both AdipoR1 and AdipoR2 was significantly less in the liver of obese (LepOb and LepDb ) mice compared to wild-type lean animals ( P < 0.001). Conclusions These data show for the first time that the adiponectin receptors AdipoR1 and AdipoR2 are expressed in the obese murine pancreas. The paucity of AdipoR1 receptors may be important when considering the role played by adipokines in the genesis of severe pancreatitis in obesity.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2008.05.006