Green tea catechins, alleviate hepatic lipidemic-oxidative injury in Wistar rats fed an atherogenic diet

• Published in: Chemico-biological interactions 2009-06, Vol.180 (1), p.10-19
• Main Authors: Ramesh, E., Jayakumar, T., Elanchezhian, R., Sakthivel, M., Geraldine, P., Thomas, P.A.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Atherogenic diet; Atherosclerosis; Camellia sinensis; Catalase - genetics; Catalase - metabolism; Catechin - administration & dosage; Catechin - pharmacology; Diet, Atherogenic; Epigallocatechin gallate; Green tea catechins; Lipid Peroxidation - drug effects; Liver - enzymology; Liver - injuries; Male; mRNA expression; Oxidative Stress; Plant Extracts - administration & dosage; Plant Extracts - pharmacology; Plant Leaves - chemistry; Rats; Rats, Wistar; RNA, Messenger - metabolism
• ISSN: 0009-2797; 1872-7786
• DOI: 10.1016/j.cbi.2009.02.013

In the present study, the efficacy of green tea catechins (GTC from the plant Camellia sinensis), with epigallocatechin gallate (EGCG), as the major component, was studied in relation to hepatic oxidative abnormalities in atherosclerotic rats. When male albino Wistar rats were fed an atherogenic diet for 30 days and then treated with saline for 7 or 15 days, there was a significant decline in hepatic mean activities of antioxidant enzymes (catalase, superoxide dismutase, glutathione peroxidase and glutathione-S-transferase), and non-enzymatic antioxidants (reduced glutathione, vitamins C and E) while there was a significant elevation in the mean level of hepatic malondialdehyde (MDA), in comparison to the values noted in control rats fed a normal diet. In addition, a concomitant increase in the activities of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) was noted, when compared to the values in control rats. Following intraperitoneal administration of GTC (100 mg/kg) for 7 or 15 days to rats fed the atherogenic diet, significantly higher mean activities of enzymatic and non-enzymatic antioxidants and lower mean levels of MDA in hepatic tissue and lower mean activities of AST, ALT, ALP and LDH in serum were observed, compared to the values in the rats fed the atherogenic diet and treated with saline. Histopathological studies were performed to provide direct evidence of the atherogenic diet-induced hepatic changes and of the hepatoprotective effect of GTC. These results suggest that EGCG as a major component of green tea catechins may protect against the hepatic abnormalities occurring in Wistar rats fed an atherogenic diet.