Massachusetts Live Vaccination Protects Against a Novel Infectious Bronchitis Virus S1 Genotype DMV/5642/06

Four infectious bronchitis virus (IBV) isolates were recovered from commercial broiler chicken flocks located on the Delmarva Peninsula (east coast of the United States) in the spring of 2006. Sequence analysis of the S1 subunit of the spike glycoprotein gene showed the four isolates were highly rel...

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Bibliographic Details
Published in:Avian diseases 2009-03, Vol.53 (1), p.119-123
Main Authors: Wood, M. K, Ladman, B. S, Preskenis, L. A, Pope, C. R, Bautista, D. A, Gelb, J
Format: Article
Language:English
Subjects:
amino acid sequences
Amino acids
Animals
broiler chickens
Bronchitis
challenge
chicken
Chickens
Coronavirus Infections - prevention & control
Coronavirus Infections - veterinary
Coronavirus Infections - virology
Delmarva
Genes, Viral - genetics
Genotype
Genotypes
glycoproteins
histopathology
IBV
Infectious bronchitis virus
Infectious bronchitis virus - classification
Infectious bronchitis virus - genetics
Infectious bronchitis virus - pathogenicity
Lesions
live vaccines
Massachusetts
microbial genetics
molecular sequence data
nucleotide sequences
Nucleotides
Poultry Diseases - prevention & control
Poultry Diseases - virology
Research Notes
S1 genotype sequence
sequence analysis
strain differences
strains
Trachea
Vaccination
variant
vertebrate viruses
Viral Vaccines - immunology
Viruses
Citations: Items that cite this one
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Description
Summary:Four infectious bronchitis virus (IBV) isolates were recovered from commercial broiler chicken flocks located on the Delmarva Peninsula (east coast of the United States) in the spring of 2006. Sequence analysis of the S1 subunit of the spike glycoprotein gene showed the four isolates were highly related to each other (≥99.6% nucleotide identity; ≥98.9% amino acid identity). Basic local alignment search tool analysis indicated the highest S1 amino acid identity of isolate DMV/5642/06, typical of the four Delmarva (DMV) isolates, was to CA/1737/04, an isolate obtained from broilers in California in 2004. A pathogenicity study conducted, using two-week-old commercial broilers, showed that DMV/5642/06 caused respiratory but not renal (kidney) disease. A vaccination–challenge study in three-week-old specific-pathogen-free leghorn chickens demonstrated that a commercial live attenuated IBV vaccine containing the Massachusetts strain conferred protection against challenge with DMV/5642/06 based on virus reisolation attempts and microscopic pathology.
ISSN:0005-2086
1938-4351
DOI:10.1637/8454-082108-ResNote.1