Frequent overexpression of CADM1/IGSF4 in lung adenocarcinoma

We reported comprehensive screening for antigens (Ags) overexpressed on various carcinomas via isolation of human monoclonal antibodies (mAbs) that may be therapeutic in a previous paper (Proc. Natl. Acad. Sci. USA 105, 7287-7292, 2008). Twenty-one distinct Ags highly expressed on several carcinomas...

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Published in:Biochemical and biophysical research communications 2009-06, Vol.383 (4), p.480-484
Main Authors: Kitamura, Yuka, Kurosawa, Gene, Tanaka, Miho, Sumitomo, Mariko, Muramatsu, Chiho, Eguchi, Keiko, Akahori, Yasushi, Iba, Yoshitaka, Tsuda, Hiroyuki, Sugiura, Mototaka, Hattori, Yoshinobu, Kurosawa, Yoshikazu
Format: Article
Language:English
Subjects:
Adenocarcinoma - immunology
Amino Acid Sequence
Antibodies, Monoclonal - immunology
Antigens, Neoplasm - biosynthesis
Antigens, Neoplasm - immunology
Cell Adhesion Molecule-1
Cell Adhesion Molecules
Epitopes - immunology
Human monoclonal antibodies
Humans
Immunoglobulins - biosynthesis
Immunoglobulins - immunology
Immunohistochemical analysis
Lung Neoplasms - immunology
Membrane Proteins - biosynthesis
Membrane Proteins - immunology
Tumor suppressor
Tumor Suppressor Proteins - biosynthesis
Tumor Suppressor Proteins - immunology
Tumor-associated antigens
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Summary:We reported comprehensive screening for antigens (Ags) overexpressed on various carcinomas via isolation of human monoclonal antibodies (mAbs) that may be therapeutic in a previous paper (Proc. Natl. Acad. Sci. USA 105, 7287-7292, 2008). Twenty-one distinct Ags highly expressed on several carcinomas were identified and 356 mAbs with unique sequences turned out to bind to one of the 21 Ags. Among them CADM1/IGSF4 which had been originally referred to as tumor suppressor lung cancer 1 (TSLC1) was included. Therefore we examined the expression of CADM1 in lung cancers in this study. Eight different anti CADM1 mAbs were used for immunohistochemical analysis of 29 fresh lung cancer specimens. Staining patterns were categorized to six groups based on the extent of positive staining and the localization of stained portions. While overexpression of CADM1 was observed on the cell surface of adenocarcinomas at a high frequency, around 60%, positive stainings were rarely observed on that of other lung carcinomas including squamous cell carcinomas. Moreover, some clones among the eight mAbs gave different staining patterns from those by the other clones against the same fresh specimen, suggesting presence of variant forms of CADM1 differentiated by mAbs.
ISSN:0006-291X
1090-2104
DOI:10.1016/j.bbrc.2009.04.039