Involvement of the S100B in cAMP-induced cytoskeleton remodeling in astrocytes: a study using TRTK-12 in digitonin-permeabilized cells

• Published in: Cellular and molecular neurobiology 2004-12, Vol.24 (6), p.833-840
• Main Authors: Frizzo, Juliana K, Tramontina, Ana Carolina, Tramontina, Francine, Gottfried, Carmem, Leal, Rodrigo B, Donato, Rosario, Gonçalves, Carlos-Alberto
• Format: Article
• Language: English
• Subjects: Animals; Astrocytes - drug effects; Astrocytes - metabolism; CapZ Actin Capping Protein; Cells, Cultured; Cyclic AMP - metabolism; Cyclic AMP - pharmacology; Cytoskeleton - drug effects; Cytoskeleton - metabolism; Digitonin - metabolism; Digitonin - pharmacology; Dose-Response Relationship, Drug; Nerve Growth Factors - metabolism; Nerve Growth Factors - pharmacology; Oligopeptides - metabolism; Oligopeptides - pharmacology; Peptide Fragments; Permeability - drug effects; Rats; Rats, Wistar; S100 Calcium Binding Protein beta Subunit; S100 Proteins - metabolism; S100 Proteins - pharmacology
• ISSN: 0272-4340; 1573-6830
• DOI: 10.1007/s10571-004-6922-y

1. Stellation of astrocytes in culture involves a complex rearrangement of microfilaments, intermediate filaments, and microtubules, which reflects in part the plasticity of these cells observed during development or after injury. 2. An astrocytic calcium-binding protein, S100B, has been implicated in the regulation of plasticity due to its ability to interact with cytoskeletal proteins. 3. We used digitonin-permeabilized astrocytes to introduce TRTK-12, a peptide that binds to the C-terminal of S100B and blocks its interaction with cytoskeletal proteins. 4. TRTK-12 was able to block cAMP-induced astrocyte stellation and this effect was dependent on the concentration of the peptide. These results support the idea that S100B has a modulatory role on astrocyte morphology.