Production of Calcium-Stearate by Lipase Using Hydrogenated Beef Tallow

• Published in: Applied biochemistry and biotechnology 2009-05, Vol.157 (2), p.278-284
• Main Authors: Lee, Hyang-Bok, Kwon, Jin-Soo, Kim, Young-Bum, Kim, Eun-Ki
• Format: Article
• Language: English
• Subjects: Acid production; Animals; beef; Biochemistry; Bioengineering; Biological and medical sciences; Biotechnology; Calcium; Calcium hydroxide; calcium stearate; Cattle; Chemistry; Chemistry and Materials Science; enzymatic treatment; enzyme substrates; Enzymes; Fats - chemistry; Fats - metabolism; Fatty acids; feed additives; Fundamental and applied biological sciences. Psychology; High temperature; hydrogenation; Hydrogenation - drug effects; Hydrolysis - drug effects; Lipase - metabolism; Meat; optimization; Stearic Acids - chemistry; Stearic Acids - metabolism; tallow; temperature; Time Factors; triacylglycerol lipase; Water - pharmacology
• ISSN: 0273-2289; 1559-0291
• DOI: 10.1007/s12010-008-8254-8

Calcium-stearate has been traditionally produced by chemical methods, producing wastes and requiring high energy because of high temperature operation. To achieve enzymatic production of calcium-stearate at unfavorable conditions, i.e., pH 10 and 60 °C, suitable lipase was selected and reaction conditions were optimized using calcium hydroxide and hydrogenated beef tallow as substrates. Under optimum conditions, 95% of beef tallow, in 2.5 h, was converted into calcium-stearate by using commercial lipase SDL 451. Investigation of the time-course reaction revealed that fatty acid was initially produced by lipase, followed by conversion into calcium-stearate. The fatty acid production rate was faster than that of the conversion into calcium-stearate at the beginning of the reaction. Alkaline pH, originating from the addition of calcium hydroxide, increased the converting reaction. This is the first report demonstrating that chemical production of calcium-stearate can be replaced by enzymatic reaction, thereby creating a cleaner process.