Experimental transmission and tissue tropism of Macrobrachium rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV)

White tail disease (WTD) was found to be a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. The causative organisms have been identified as M. rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV). Experimentally transmitted to healthy animals,...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2004-12, Vol.62 (3), p.191-196
Main Authors: HAMEED, A. S, YOGANANDHAN, K, WIDADA, J. Sri, BONAMI, J. R
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Animals
Aquaculture
Biological and medical sciences
Crustacea
Disease Transmission, Infectious
DNA Primers
Fundamental and applied biological sciences. Psychology
India
Invertebrate aquaculture
Macrobrachium rosenbergii
Mortality
Nodaviridae - pathogenicity
Nodavirus
Palaemonidae - virology
Reverse Transcriptase Polymerase Chain Reaction
Viral Load
Virion - pathogenicity
Viscera - virology
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Summary:White tail disease (WTD) was found to be a serious problem in hatcheries and nursery ponds of Macrobrachium rosenbergii in India. The causative organisms have been identified as M. rosenbergii nodavirus (MrNV) and its associated extra small virus (XSV). Experimentally transmitted to healthy animals, they caused 100% mortality in post-larvae but failed to cause mortality in adult prawns. The RT-PCR assay revealed the presence of both viruses in moribund post-larvae and in gill tissue, head muscle, stomach, intestine, heart, hemolymph, pleopods, ovaries and tail muscle, but not in eyestalks or the hepatopancreas of experimentally infected adult prawns. The presence of these viruses in ovarian tissue indicates the possibility of vertical transmission. Pleopods have been found to be a suitable organ for detecting these viruses in brooders using the RT-PCR technique.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao062191