Importance of the porcine ADAM3 disintegrin domain in sperm-egg interaction

In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have id...

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Bibliographic Details
Published in:Journal of Reproduction and Development 2009, Vol.55(2), pp.156-162
Main Authors: Kim, E.(Korea Research Inst. of Bioscience and Biotechnology, Chungbuk (Korea R.)), Park, K.E, Kim, J.S, Baek, D.C, Lee, J.W, Lee, S.R, Kim, M.S, Kim, S.H, Kim C.S, Koo, D.B, Kang, H.S, Ryoo, Z.Y, Chang, K.T
Format: Article
Language:English
Subjects:
A disintegrin and metalloprotease (ADAM)
ADAM Proteins - genetics
ADAM Proteins - physiology
Amino Acid Sequence
Animals
Blotting, Southern - veterinary
Blotting, Western - veterinary
CERDO
Disintegrin domain
DNA - chemistry
DNA - genetics
ESPERMATOZOO
Female
Fertilization in Vitro - veterinary
Gene Dosage
Male
Molecular Sequence Data
OVA
OVULE
OVULO
Polymerase Chain Reaction - veterinary
PORCIN
Porcine
PROTEASAS
PROTEASE
PROTEASES
Protein Isoforms
Protein Structure, Tertiary
PROTEINAS
PROTEINE
PROTEINS
Sequence Alignment
Sperm-egg interaction
Sperm-Ovum Interactions - physiology
SPERMATOZOA
SPERMATOZOIDE
SWINE
Swine - genetics
Swine - physiology
TESTES
TESTICULE
TESTICULOS
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Summary:In the mouse, ADAM3, a well-characterized testis-specific protein of the A disintegrin and metalloprotease (ADAM) family, has a crucial role in fertilization by mediating sperm binding to the egg zona pellucida. However, little is known about ADAM3 in other species, such as domestic pigs. We have identified porcine ADAM3 and analyzed the protein. RT-PCR and trypsinization of sperm surface proteins revealed that porcine ADAM3 is expressed at high levels in the testis and on the sperm surface. Furthermore, an IVF inhibition assay with a recombinant porcine ADAM3 disintegrin domain showed that treatment of the disintegrin domain effectively prevented pig sperm-egg interactions. In the present study, we demonstrated the presence of ADAM3a and ADAM3b molecules in the pig and examined their roles in fertilization.
ISSN:0916-8818
1348-4400
DOI:10.1262/jrd.20134