Growth phase-dependant enzyme profile of pyruvate catabolism and end-product formation in Clostridium thermocellum ATCC 27405

End-product synthesis and enzyme activities involved in pyruvate catabolism, H2 synthesis, and ethanol production in mid-log (OD600∼0.25), early stationary (OD600∼0.5), and stationary phase (OD600∼0.7) cell extracts were determined in Clostridium thermocellum ATCC 27405 grown in batch cultures on ce...

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Bibliographic Details
Published in:Journal of biotechnology 2009-03, Vol.140 (3-4), p.169-175
Main Authors: Rydzak, Thomas, Levin, David B., Cicek, Nazim, Sparling, Richard
Format: Article
Language:English
Subjects:
acetic acid
Biological and medical sciences
biosynthesis
Biotechnology
carbohydrate metabolism
carbon dioxide
cellobiose
cellulolytic microorganisms
Clostridium thermocellum
Clostridium thermocellum - enzymology
Clostridium thermocellum - growth & development
Clostridium thermocellum - metabolism
Consolidated bioprocessing
enzyme activity
Enzyme activity shift
enzymes
Ethanol - metabolism
ethanol production
Ethanol synthesis
formic acid
Fundamental and applied biological sciences. Psychology
gas production (biological)
Gene Expression Regulation, Bacterial - physiology
growth phase
hydrogen
Hydrogen - metabolism
Hydrogen synthesis
metabolism
microbial physiology
Pyruvate catabolism
pyruvic acid
Pyruvic Acid - metabolism
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Summary:End-product synthesis and enzyme activities involved in pyruvate catabolism, H2 synthesis, and ethanol production in mid-log (OD600∼0.25), early stationary (OD600∼0.5), and stationary phase (OD600∼0.7) cell extracts were determined in Clostridium thermocellum ATCC 27405 grown in batch cultures on cellobiose. Carbon dioxide, hydrogen, ethanol, acetate and formate were major end-products and their production paralleled growth and cellobiose consumption. Lactate dehydrogenase, pyruvate:formate lyase, pyruvate:ferredoxin oxidoreductase, methyl viologen-dependant hydrogenase, ferredoxin-dependant hydrogenase, NADH-dependant hydrogenase, NADPH-dependant hydrogenase, NADH-dependant acetaldehyde dehydrogenase, NADH-dependant alcohol dehydogenase, and NADPH-dependant alcohol dehydrogenase activities were detected in all extracts, while pyruate dehydrogenase and formate dehydrogenase activities were not detected. All hydrogenase activities decreased (2–12-fold) as growth progressed from early exponential to stationary phase. Alcohol dehydrogenase activities fluctuated only marginally (
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2009.01.022