Expression of tracheal antimicrobial peptide in bovine mammary epithelial cells

The production of antimicrobial peptides is an important key of innate immunity. Tracheal antimicrobial peptide (TAP) expression has been reported in bovine tracheal epithelial cells and it can be modulated by bacterial infection or bacterial components. In mammary gland TAP expression has been repo...

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Bibliographic Details
Published in:Research in veterinary science 2009-08, Vol.87 (1), p.59-63
Main Authors: López-Meza, Joel E., Gutiérrez-Barroso, Angelina, Ochoa-Zarzosa, Alejandra
Format: Article
Language:English
Subjects:
Acetyl salicylic acid
Animals
Antimicrobial agents
Antimicrobial Cationic Peptides - genetics
Antimicrobial Cationic Peptides - metabolism
antimicrobial peptides
aspirin
Aspirin - pharmacology
Bacteria
Bacterial infections
biochemical pathways
Cattle
cattle diseases
Cells, Cultured
Defense mechanisms
epithelial cells
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Female
Gene Expression Regulation - physiology
gentamicin
in vitro studies
Mammary epithelium
mammary glands
Mammary Glands, Animal - cytology
messenger RNA
polymerase chain reaction
Prolactin
Prolactin - pharmacology
protein synthesis
Proteins
retrotranscription
Rodents
Staphylococcus aureus
Staphylococcus infections
Tracheal antimicrobial peptide
tracheal antimicrobial peptides
Veterinary medicine
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Summary:The production of antimicrobial peptides is an important key of innate immunity. Tracheal antimicrobial peptide (TAP) expression has been reported in bovine tracheal epithelial cells and it can be modulated by bacterial infection or bacterial components. In mammary gland TAP expression has been reported, but the cell type that produces it is unknown. The objective of this work was to evaluate if bovine mammary epithelial cells (bMEC) express TAP mRNA, and evaluate the regulation of its expression in response to Staphylococcus aureus infection, bovine prolactin (bPRL) or acetyl salicylic acid (ASA). By retrotranscription and PCR, we demonstrated that bMEC express TAP mRNA. bMEC infected with live S. aureus down-regulates TAP expression, whereas the challenge with gentamicin-killed S. aureus up-regulates it. Also, bPRL do not significantly modify TAP expression, but in the presence of 5 mM ASA it was down-regulated, suggesting that nuclear factor κB (NF-κB) pathway can be involved in its regulation.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2008.12.005