Patterned networks of mouse hippocampal neurons on peptide-coated gold surfaces

Patterned networks of hippocampal neurons were generated on peptide-coated gold substrates prepared by microscope projection photolithography and microcontact printing. A 19 amino acid peptide fragment of laminin A (PA22-2) that includes the IKVAV cell adhesion domain was used to direct patterns of...

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Bibliographic Details
Published in:Biomaterials 2005-03, Vol.26 (8), p.883-889
Main Authors: Heller, Daniel A., Garga, Veronika, Kelleher, Keith J., Lee, Tai-Chou, Mahbubani, Sunil, Sigworth, Laura A., Lee, T.Randall, Rea, Michael A.
Format: Article
Language:English
Subjects:
Animals
Coated Materials, Biocompatible
Electrophysiology
Gold
Hippocampus
Hippocampus - cytology
Hippocampus - physiology
Membrane Potentials - physiology
Mice
Microcontact printing
Microscopy, Atomic Force
Neural network
Neuron
Neurons - cytology
Neurons - physiology
PA22-2
Patch-Clamp Techniques
Peptides
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Summary:Patterned networks of hippocampal neurons were generated on peptide-coated gold substrates prepared by microscope projection photolithography and microcontact printing. A 19 amino acid peptide fragment of laminin A (PA22-2) that includes the IKVAV cell adhesion domain was used to direct patterns of cell adhesion in primary culture. Microscale grid patterns of peptide were deposited on gold-coated glass cover slips by soft lithography using “stamps” fashioned from polydimethylsiloxane. Strong coordination bonding between gold atoms on the surface and the sulfur atoms of the N-terminal cysteine residues supported stable adhesion of the peptide, which was confirmed by immunofluorescence using anti-IKVAV antiserum. Dispersed hippocampal cells isolated from neonatal mouse pups were grown on peptide-patterned gold substrates for 7 days. Neurons preferentially adhered to peptide-coated regions of the gold surface and restricted their processes to the peptide patterns. Whole cell recordings of neurons grown in patterned arrays revealed an average membrane potential of −50 mV, as well as the presence of voltage-gated ion conductances. Peptide-modified gold surfaces serve as convenient and effective substrates for growing ordered neural networks that are compatible with existing multi-electrode array recording technology.
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2004.03.029