A zoospore inhibition technique to evaluate the activity of antifungal compounds against Batrachochytrium dendrobatidis and unsuccessful treatment of experimentally infected green tree frogs ( Litoria caerulea) by fluconazole and benzalkonium chloride

Effective and safe treatments of chytridiomycosis in amphibians, caused by Batrachochytrium dendrobatidis, are needed to help prevent mortality in captive programs for threatened species, to reduce the risk of spread, and to better manage the disease in threatened populations. We describe a simple m...

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Bibliographic Details
Published in:Research in veterinary science 2009-08, Vol.87 (1), p.106-110
Main Authors: Berger, Lee, Speare, Rick, Marantelli, Gerry, Skerratt, Lee F.
Format: Article
Language:English
Subjects:
Animals
antifungal agents
Antifungal Agents - therapeutic use
Antifungal testing
Anura - microbiology
Batrachochytrium dendrobatidis
benzalkonium chloride
Benzalkonium Compounds - therapeutic use
captive animals
Chytridiomycosis
Chytridiomycota - drug effects
Disinfection & disinfectants
dose response
Drug dosages
Endangered & extinct species
enilconazole
fluconazole
Fluconazole - therapeutic use
Frogs
Hylidae
itraconazole
Litoria caerulea
mercurochrome
Methods
methylene blue
Microbiology
minimum inhibitory concentration
mycoses
Mycoses - drug therapy
Mycoses - veterinary
sodium chloride
Studies
threatened species
Treatment
Veterinary medicine
virkon
Water quality
zoospores
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Description
Summary:Effective and safe treatments of chytridiomycosis in amphibians, caused by Batrachochytrium dendrobatidis, are needed to help prevent mortality in captive programs for threatened species, to reduce the risk of spread, and to better manage the disease in threatened populations. We describe a simple method to determine minimum inhibitory concentrations (MICs) of antifungal agents that involves adding zoospores to various drug concentrations in 96 well plates and microscopic observation after four days. We report results from testing 10 commercially available antifungal compounds: benzalkonium chloride (
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2008.11.005