in vivo synthesis and accumulation of lectin in developing seeds of black gram (Vigna mungo L. Hepper)

Black gram (Vigna mungo L. Hepper) seed contains two D-galactose-specific lectin species, BGL-I and BGL-II, identified on the basis of elution from ion exchange column and immunochemical cross-reactivity. BGL-I consisted of two monomeric lectins, BGL-I-1 and BGL-1-2, of relative molecular weights 94...

Full description

Saved in:
Bibliographic Details
Published in:Plant foods for human nutrition (Dordrecht) 2004, Vol.59 (3), p.123-128
Main Authors: Suseelan, K.N, Mitra, R, Bhatia, C.R, Gopalakrishna, T
Format: Article
Language:English
Subjects:
Biological and medical sciences
biosynthesis
black gram
Food Analysis
Food industries
Fundamental and applied biological sciences. Psychology
Immunodiffusion - methods
lectins
Molecular Weight
Phaseolus - chemistry
Plant Lectins - analysis
Plant Lectins - biosynthesis
seed development
seeds
Seeds - chemistry
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Black gram (Vigna mungo L. Hepper) seed contains two D-galactose-specific lectin species, BGL-I and BGL-II, identified on the basis of elution from ion exchange column and immunochemical cross-reactivity. BGL-I consisted of two monomeric lectins, BGL-I-1 and BGL-1-2, of relative molecular weights 94 and 89 kDa, respectively. BGL-II is another monomeric lectin with a molecular weight of 83 kDa. The in vivo synthesis studies using pulse-chase experiment showed that BGL-II lectin was synthesized as early as 14 days after flowering (DAF). The 94-kDa BGL-I-1 lectin was synthesized around 17 DAF. There was no cotranslational or posttranslational modification of the lectin proteins. The amount of lectin in developing seeds was determined by radial immunodiffusion assay technique. The maximum amount of lectin per seed was found at 28 DAF.
ISSN:0921-9668
1573-9104
DOI:10.1007/s11130-004-0050-1