In vitro assessment of progesterone and prostaglandin e(2) production by the corpus luteum in cattle following pharmacological synchronization of estrus

We studied the secretory function of the corpus luteum (CL) in cows following different estrus synchronization protocols. Estrus was synchronized using one (n=4) or two injections (n=5) of prostaglandin F(2alpha) (PGF(2alpha); dinoprost), two injections of different analogues of PGF(2alpha) (aPGF(2a...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of reproduction and development 2009-04, Vol.55 (2), p.170-176
Main Authors: Skarzynski, Dariusz Jan, Siemieniuch, Marta Jolanta, Pilawski, Wojciech, Woclawek Potocka, Izabela, Bah, Mamadou Moussa, Majewska, Magdalena, Jaroszewski, Jerzy Jan
Format: Article
Language:English
Subjects:
Animals
Cattle
Cloprostenol - pharmacology
Corpus Luteum - metabolism
Dinoprost - pharmacology
Dinoprostone - biosynthesis
Dinoprostone - blood
Dinoprostone - pharmacology
Estrus Synchronization - methods
Female
In Vitro Techniques
Luteinizing Hormone - pharmacology
Pilot Projects
Progesterone - biosynthesis
Progesterone - metabolism
Prostaglandins F, Synthetic - pharmacology
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We studied the secretory function of the corpus luteum (CL) in cows following different estrus synchronization protocols. Estrus was synchronized using one (n=4) or two injections (n=5) of prostaglandin F(2alpha) (PGF(2alpha); dinoprost), two injections of different analogues of PGF(2alpha) (aPGF(2alpha)), luprostiol (n=5) and cloprostenol (n=5), at eleven-day intervals, a gestagen implant (norgestomet, n=5, for 10 days) or norgestomet together with a subsequent dinoprost injection on the day of implant removal (n=5). CL samples were collected by ovariectomy on Day 7-8 of the estrous cycle. Luteal strips were stimulated with LH (100 ng/ml) or prostaglandin E(2) (PGE(2), 10(-6)M) for 24 h in culture media. The progesterone (P(4)) and PGE(2) concentrations in the media were measured by enzyme immunoassay. In the control CL (spontaneous estrus; n=5), LH and PGE(2) stimulated P(4) and PGE(2) (P
ISSN:0916-8818