Effectiveness of small interfering RNA (siRNA) against the Mcl-1 gene in a canine mammary gland tumor cell line

The effect of down-regulation of Mcl-1 expression by small interfering RNA (siRNA) against the canine Mcl-1 gene on apoptosis was investigated by transfecting CF33 (canine mammary gland tumor cell line) with siRNA using cationic liposomes. The siRNA against canine Mcl-1 increased the rate of apoptot...

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Bibliographic Details
Published in:Research in veterinary science 2009-08, Vol.87 (1), p.64-66
Main Authors: Kano, Rui, Yano, Takayuki, Nagamatsu, Kota, Maruyama, Haruhiko, Kamata, Hiroshi, Hasegawa, Atsuhiko
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Canine mammary gland tumor
cationic liposomes
Cell Line, Tumor
cell lines
Cells
Dogs
Female
gene expression
Gene Expression Regulation, Neoplastic - drug effects
Genes
mammary glands
Mammary Glands, Animal - cytology
mammary neoplasms (animal)
Mcl-1
Mcl-1 gene
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
polymerase chain reaction
Proteins
real time polymerase chain reaction
RNA
RNA, Small Interfering - pharmacology
small interfering RNA
Small interfering RNA (siRNA)
Tumors
Veterinary medicine
Western blotting
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Summary:The effect of down-regulation of Mcl-1 expression by small interfering RNA (siRNA) against the canine Mcl-1 gene on apoptosis was investigated by transfecting CF33 (canine mammary gland tumor cell line) with siRNA using cationic liposomes. The siRNA against canine Mcl-1 increased the rate of apoptotic cells and decreased the numbers of viable cells. Further, sequence-specific down-regulation of Mcl-1 expression was measured by real time-PCR and Western blot analysis. The siRNA directed against the Mcl-1 gene reduced both the mRNA and protein expression in the CF33. Our study suggests the importance of Mcl-1 in canine mammary tumors for inducing apoptosis and reinforces using Mcl-1 as a putative therapeutic target in canine mammary gland tumor.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2008.12.006