Fish as bioreactors: transgene expression of human coagulation factor VII in fish embryos

A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression...

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Bibliographic Details
Published in:Marine biotechnology (New York, N.Y.) N.Y.), 2004-10, Vol.6 (5), p.485-492
Main Authors: Hwang, Gyulin, Müller, Ferenc, Rahman, M Aziz, Williams, Darren W, Murdock, Paul J, Pasi, K John, Goldspink, Geoffrey, Farahmand, Hamid, Maclean, Norman
Format: Article
Language:English
Subjects:
Animals
Bioreactors
Blood Coagulation - drug effects
Coagulation
Cytomegalovirus - genetics
Danio rerio
DNA, Complementary - genetics
Embryos
Enzyme-Linked Immunosorbent Assay
Factor VII - genetics
Factor VII - metabolism
Factor VII - pharmacology
Fish
Fishes - metabolism
Genetic Vectors - genetics
Human cytomegalovirus
Humans
Microinjections
Photometry
Promoter Regions, Genetic - genetics
Proteins
Tilapia
Transgenes - genetics
Zygote - metabolism
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Summary:A plasmid containing human coagulation factor VII (hFVII) complementary DNA regulated by a cytomegalovirus promoter was microinjected into fertilized eggs of zebrafish, African catfish, and tilapia. The active form of hFVll was detected in the fish embryos by various assays. This positive expression of human therapeutic protein in fish embryos demonstrates the possibility of exploitation of transgenic fish as bioreactors.
ISSN:1436-2228
1436-2236
DOI:10.1007/s10126-004-3121-2