Loading…
Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway
Background and Objective: While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells...
Saved in:
Published in: | Journal of periodontal research 2009-06, Vol.44 (3), p.289-296 |
---|---|
Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | cdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93 |
---|---|
cites | cdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93 |
container_end_page | 296 |
container_issue | 3 |
container_start_page | 289 |
container_title | Journal of periodontal research |
container_volume | 44 |
creator | Matsuzawa, M. Sheu, T.-J. Lee, Y.-J. Chen, M. Li, T.-F. Huang, C. T. Holz, J. D. Puzas, J. E. |
description | Background and Objective: While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells.
Material and Methods: To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene.
Results: In these in vitro models, we showed that amelogenin can activate β‐catenin signaling.
Conclusion: Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone. |
doi_str_mv | 10.1111/j.1600-0765.2008.01091.x |
format | article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67277641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20545367</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</originalsourceid><addsrcrecordid>eNqNkM9u1DAQhy1ERZfCK6Bc4JbU_x0fOEBV2tJVSytQj5bXGW-9ZJMlduhuH4sH4ZlIuqvlCHPxWPP9xtaHUEZwQYY6XhREYpxjJUVBMS4LTLAmxfoZmuwHz9EEY0pzxkt-iF7GuMDDXSr9Ah0SzSXlZTlBl1_6ZFP4CVkM88bWoZln1qXQNlnrM7uEup1DE5qsT6EOjxCzdA_ZXZOOf__KnU1Ps5VN9w928wodeFtHeL07j9C3T6dfT87z6fXZxcmHae64lCSvBNPAHeGCgGBEcY1BAgOJZ8wJboVWuPK-qoTwVDohndfYl7JUM0w9aHaE3m33rrr2Rw8xmWWIDuraNtD20UhFlZKc_BOkWHDBpBrAcgu6ro2xA29WXVjabmMINqNxszCjWDOKNaNx82TcrIfom90b_WwJ1d_gTvEAvN0BNjpb-842LsQ9RwkrhSZy4N5vuYdQw-a_P2A-356O3ZDPt_kQE6z3edt9H4QwJczd1Zlhtzc35x_F1Cj2B939q04</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20545367</pqid></control><display><type>article</type><title>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</title><source>Wiley-Blackwell Read & Publish Collection</source><creator>Matsuzawa, M. ; Sheu, T.-J. ; Lee, Y.-J. ; Chen, M. ; Li, T.-F. ; Huang, C. T. ; Holz, J. D. ; Puzas, J. E.</creator><creatorcontrib>Matsuzawa, M. ; Sheu, T.-J. ; Lee, Y.-J. ; Chen, M. ; Li, T.-F. ; Huang, C. T. ; Holz, J. D. ; Puzas, J. E.</creatorcontrib><description>Background and Objective: While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells.
Material and Methods: To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene.
Results: In these in vitro models, we showed that amelogenin can activate β‐catenin signaling.
Conclusion: Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2008.01091.x</identifier><identifier>PMID: 19462488</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Alveolar Process - metabolism ; amelogenin ; Amelogenin - biosynthesis ; Amelogenin - genetics ; Amelogenin - physiology ; Animals ; beta Catenin - biosynthesis ; beta Catenin - metabolism ; beta-Galactosidase - biosynthesis ; Biological and medical sciences ; bone formation ; cell signaling ; Cells, Cultured ; Dentistry ; Gene Expression ; Genes, Reporter ; Humans ; Medical sciences ; Mice ; Mice, Transgenic ; osteoblast ; Osteoblasts - metabolism ; Otorhinolaryngology. Stomatology ; periodontal ligament ; Periodontal Ligament - cytology ; Periodontal Ligament - metabolism ; Recombinant Proteins - pharmacology ; Signal Transduction - physiology ; TCF Transcription Factors - metabolism ; Tooth Root - metabolism ; Transfection ; Wnt Proteins - metabolism ; β-catenin</subject><ispartof>Journal of periodontal research, 2009-06, Vol.44 (3), p.289-296</ispartof><rights>2009 John Wiley & Sons A/S</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</citedby><cites>FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21385916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19462488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuzawa, M.</creatorcontrib><creatorcontrib>Sheu, T.-J.</creatorcontrib><creatorcontrib>Lee, Y.-J.</creatorcontrib><creatorcontrib>Chen, M.</creatorcontrib><creatorcontrib>Li, T.-F.</creatorcontrib><creatorcontrib>Huang, C. T.</creatorcontrib><creatorcontrib>Holz, J. D.</creatorcontrib><creatorcontrib>Puzas, J. E.</creatorcontrib><title>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objective: While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells.
Material and Methods: To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene.
Results: In these in vitro models, we showed that amelogenin can activate β‐catenin signaling.
Conclusion: Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.</description><subject>Alveolar Process - metabolism</subject><subject>amelogenin</subject><subject>Amelogenin - biosynthesis</subject><subject>Amelogenin - genetics</subject><subject>Amelogenin - physiology</subject><subject>Animals</subject><subject>beta Catenin - biosynthesis</subject><subject>beta Catenin - metabolism</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>bone formation</subject><subject>cell signaling</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Gene Expression</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>osteoblast</subject><subject>Osteoblasts - metabolism</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Signal Transduction - physiology</subject><subject>TCF Transcription Factors - metabolism</subject><subject>Tooth Root - metabolism</subject><subject>Transfection</subject><subject>Wnt Proteins - metabolism</subject><subject>β-catenin</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkM9u1DAQhy1ERZfCK6Bc4JbU_x0fOEBV2tJVSytQj5bXGW-9ZJMlduhuH4sH4ZlIuqvlCHPxWPP9xtaHUEZwQYY6XhREYpxjJUVBMS4LTLAmxfoZmuwHz9EEY0pzxkt-iF7GuMDDXSr9Ah0SzSXlZTlBl1_6ZFP4CVkM88bWoZln1qXQNlnrM7uEup1DE5qsT6EOjxCzdA_ZXZOOf__KnU1Ps5VN9w928wodeFtHeL07j9C3T6dfT87z6fXZxcmHae64lCSvBNPAHeGCgGBEcY1BAgOJZ8wJboVWuPK-qoTwVDohndfYl7JUM0w9aHaE3m33rrr2Rw8xmWWIDuraNtD20UhFlZKc_BOkWHDBpBrAcgu6ro2xA29WXVjabmMINqNxszCjWDOKNaNx82TcrIfom90b_WwJ1d_gTvEAvN0BNjpb-842LsQ9RwkrhSZy4N5vuYdQw-a_P2A-356O3ZDPt_kQE6z3edt9H4QwJczd1Zlhtzc35x_F1Cj2B939q04</recordid><startdate>200906</startdate><enddate>200906</enddate><creator>Matsuzawa, M.</creator><creator>Sheu, T.-J.</creator><creator>Lee, Y.-J.</creator><creator>Chen, M.</creator><creator>Li, T.-F.</creator><creator>Huang, C. T.</creator><creator>Holz, J. D.</creator><creator>Puzas, J. E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200906</creationdate><title>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</title><author>Matsuzawa, M. ; Sheu, T.-J. ; Lee, Y.-J. ; Chen, M. ; Li, T.-F. ; Huang, C. T. ; Holz, J. D. ; Puzas, J. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alveolar Process - metabolism</topic><topic>amelogenin</topic><topic>Amelogenin - biosynthesis</topic><topic>Amelogenin - genetics</topic><topic>Amelogenin - physiology</topic><topic>Animals</topic><topic>beta Catenin - biosynthesis</topic><topic>beta Catenin - metabolism</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>bone formation</topic><topic>cell signaling</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Gene Expression</topic><topic>Genes, Reporter</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>osteoblast</topic><topic>Osteoblasts - metabolism</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - metabolism</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Signal Transduction - physiology</topic><topic>TCF Transcription Factors - metabolism</topic><topic>Tooth Root - metabolism</topic><topic>Transfection</topic><topic>Wnt Proteins - metabolism</topic><topic>β-catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuzawa, M.</creatorcontrib><creatorcontrib>Sheu, T.-J.</creatorcontrib><creatorcontrib>Lee, Y.-J.</creatorcontrib><creatorcontrib>Chen, M.</creatorcontrib><creatorcontrib>Li, T.-F.</creatorcontrib><creatorcontrib>Huang, C. T.</creatorcontrib><creatorcontrib>Holz, J. D.</creatorcontrib><creatorcontrib>Puzas, J. E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuzawa, M.</au><au>Sheu, T.-J.</au><au>Lee, Y.-J.</au><au>Chen, M.</au><au>Li, T.-F.</au><au>Huang, C. T.</au><au>Holz, J. D.</au><au>Puzas, J. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2009-06</date><risdate>2009</risdate><volume>44</volume><issue>3</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective: While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells.
Material and Methods: To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene.
Results: In these in vitro models, we showed that amelogenin can activate β‐catenin signaling.
Conclusion: Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19462488</pmid><doi>10.1111/j.1600-0765.2008.01091.x</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0022-3484 |
ispartof | Journal of periodontal research, 2009-06, Vol.44 (3), p.289-296 |
issn | 0022-3484 1600-0765 |
language | eng |
recordid | cdi_proquest_miscellaneous_67277641 |
source | Wiley-Blackwell Read & Publish Collection |
subjects | Alveolar Process - metabolism amelogenin Amelogenin - biosynthesis Amelogenin - genetics Amelogenin - physiology Animals beta Catenin - biosynthesis beta Catenin - metabolism beta-Galactosidase - biosynthesis Biological and medical sciences bone formation cell signaling Cells, Cultured Dentistry Gene Expression Genes, Reporter Humans Medical sciences Mice Mice, Transgenic osteoblast Osteoblasts - metabolism Otorhinolaryngology. Stomatology periodontal ligament Periodontal Ligament - cytology Periodontal Ligament - metabolism Recombinant Proteins - pharmacology Signal Transduction - physiology TCF Transcription Factors - metabolism Tooth Root - metabolism Transfection Wnt Proteins - metabolism β-catenin |
title | Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T13%3A55%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Putative%20signaling%20action%20of%20amelogenin%20utilizes%20the%20Wnt/%CE%B2-catenin%20pathway&rft.jtitle=Journal%20of%20periodontal%20research&rft.au=Matsuzawa,%20M.&rft.date=2009-06&rft.volume=44&rft.issue=3&rft.spage=289&rft.epage=296&rft.pages=289-296&rft.issn=0022-3484&rft.eissn=1600-0765&rft_id=info:doi/10.1111/j.1600-0765.2008.01091.x&rft_dat=%3Cproquest_cross%3E20545367%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20545367&rft_id=info:pmid/19462488&rfr_iscdi=true |