Loading…

Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway

Background and Objective:  While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells...

Full description

Saved in:
Bibliographic Details
Published in:Journal of periodontal research 2009-06, Vol.44 (3), p.289-296
Main Authors: Matsuzawa, M., Sheu, T.-J., Lee, Y.-J., Chen, M., Li, T.-F., Huang, C. T., Holz, J. D., Puzas, J. E.
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93
cites cdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93
container_end_page 296
container_issue 3
container_start_page 289
container_title Journal of periodontal research
container_volume 44
creator Matsuzawa, M.
Sheu, T.-J.
Lee, Y.-J.
Chen, M.
Li, T.-F.
Huang, C. T.
Holz, J. D.
Puzas, J. E.
description Background and Objective:  While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. Material and Methods:  To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene. Results:  In these in vitro models, we showed that amelogenin can activate β‐catenin signaling. Conclusion:  Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.
doi_str_mv 10.1111/j.1600-0765.2008.01091.x
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67277641</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20545367</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</originalsourceid><addsrcrecordid>eNqNkM9u1DAQhy1ERZfCK6Bc4JbU_x0fOEBV2tJVSytQj5bXGW-9ZJMlduhuH4sH4ZlIuqvlCHPxWPP9xtaHUEZwQYY6XhREYpxjJUVBMS4LTLAmxfoZmuwHz9EEY0pzxkt-iF7GuMDDXSr9Ah0SzSXlZTlBl1_6ZFP4CVkM88bWoZln1qXQNlnrM7uEup1DE5qsT6EOjxCzdA_ZXZOOf__KnU1Ps5VN9w928wodeFtHeL07j9C3T6dfT87z6fXZxcmHae64lCSvBNPAHeGCgGBEcY1BAgOJZ8wJboVWuPK-qoTwVDohndfYl7JUM0w9aHaE3m33rrr2Rw8xmWWIDuraNtD20UhFlZKc_BOkWHDBpBrAcgu6ro2xA29WXVjabmMINqNxszCjWDOKNaNx82TcrIfom90b_WwJ1d_gTvEAvN0BNjpb-842LsQ9RwkrhSZy4N5vuYdQw-a_P2A-356O3ZDPt_kQE6z3edt9H4QwJczd1Zlhtzc35x_F1Cj2B939q04</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20545367</pqid></control><display><type>article</type><title>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</title><source>Wiley-Blackwell Read &amp; Publish Collection</source><creator>Matsuzawa, M. ; Sheu, T.-J. ; Lee, Y.-J. ; Chen, M. ; Li, T.-F. ; Huang, C. T. ; Holz, J. D. ; Puzas, J. E.</creator><creatorcontrib>Matsuzawa, M. ; Sheu, T.-J. ; Lee, Y.-J. ; Chen, M. ; Li, T.-F. ; Huang, C. T. ; Holz, J. D. ; Puzas, J. E.</creatorcontrib><description>Background and Objective:  While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. Material and Methods:  To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene. Results:  In these in vitro models, we showed that amelogenin can activate β‐catenin signaling. Conclusion:  Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.</description><identifier>ISSN: 0022-3484</identifier><identifier>EISSN: 1600-0765</identifier><identifier>DOI: 10.1111/j.1600-0765.2008.01091.x</identifier><identifier>PMID: 19462488</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Alveolar Process - metabolism ; amelogenin ; Amelogenin - biosynthesis ; Amelogenin - genetics ; Amelogenin - physiology ; Animals ; beta Catenin - biosynthesis ; beta Catenin - metabolism ; beta-Galactosidase - biosynthesis ; Biological and medical sciences ; bone formation ; cell signaling ; Cells, Cultured ; Dentistry ; Gene Expression ; Genes, Reporter ; Humans ; Medical sciences ; Mice ; Mice, Transgenic ; osteoblast ; Osteoblasts - metabolism ; Otorhinolaryngology. Stomatology ; periodontal ligament ; Periodontal Ligament - cytology ; Periodontal Ligament - metabolism ; Recombinant Proteins - pharmacology ; Signal Transduction - physiology ; TCF Transcription Factors - metabolism ; Tooth Root - metabolism ; Transfection ; Wnt Proteins - metabolism ; β-catenin</subject><ispartof>Journal of periodontal research, 2009-06, Vol.44 (3), p.289-296</ispartof><rights>2009 John Wiley &amp; Sons A/S</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</citedby><cites>FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=21385916$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19462488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Matsuzawa, M.</creatorcontrib><creatorcontrib>Sheu, T.-J.</creatorcontrib><creatorcontrib>Lee, Y.-J.</creatorcontrib><creatorcontrib>Chen, M.</creatorcontrib><creatorcontrib>Li, T.-F.</creatorcontrib><creatorcontrib>Huang, C. T.</creatorcontrib><creatorcontrib>Holz, J. D.</creatorcontrib><creatorcontrib>Puzas, J. E.</creatorcontrib><title>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</title><title>Journal of periodontal research</title><addtitle>J Periodontal Res</addtitle><description>Background and Objective:  While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. Material and Methods:  To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene. Results:  In these in vitro models, we showed that amelogenin can activate β‐catenin signaling. Conclusion:  Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.</description><subject>Alveolar Process - metabolism</subject><subject>amelogenin</subject><subject>Amelogenin - biosynthesis</subject><subject>Amelogenin - genetics</subject><subject>Amelogenin - physiology</subject><subject>Animals</subject><subject>beta Catenin - biosynthesis</subject><subject>beta Catenin - metabolism</subject><subject>beta-Galactosidase - biosynthesis</subject><subject>Biological and medical sciences</subject><subject>bone formation</subject><subject>cell signaling</subject><subject>Cells, Cultured</subject><subject>Dentistry</subject><subject>Gene Expression</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Transgenic</subject><subject>osteoblast</subject><subject>Osteoblasts - metabolism</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>periodontal ligament</subject><subject>Periodontal Ligament - cytology</subject><subject>Periodontal Ligament - metabolism</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Signal Transduction - physiology</subject><subject>TCF Transcription Factors - metabolism</subject><subject>Tooth Root - metabolism</subject><subject>Transfection</subject><subject>Wnt Proteins - metabolism</subject><subject>β-catenin</subject><issn>0022-3484</issn><issn>1600-0765</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNqNkM9u1DAQhy1ERZfCK6Bc4JbU_x0fOEBV2tJVSytQj5bXGW-9ZJMlduhuH4sH4ZlIuqvlCHPxWPP9xtaHUEZwQYY6XhREYpxjJUVBMS4LTLAmxfoZmuwHz9EEY0pzxkt-iF7GuMDDXSr9Ah0SzSXlZTlBl1_6ZFP4CVkM88bWoZln1qXQNlnrM7uEup1DE5qsT6EOjxCzdA_ZXZOOf__KnU1Ps5VN9w928wodeFtHeL07j9C3T6dfT87z6fXZxcmHae64lCSvBNPAHeGCgGBEcY1BAgOJZ8wJboVWuPK-qoTwVDohndfYl7JUM0w9aHaE3m33rrr2Rw8xmWWIDuraNtD20UhFlZKc_BOkWHDBpBrAcgu6ro2xA29WXVjabmMINqNxszCjWDOKNaNx82TcrIfom90b_WwJ1d_gTvEAvN0BNjpb-842LsQ9RwkrhSZy4N5vuYdQw-a_P2A-356O3ZDPt_kQE6z3edt9H4QwJczd1Zlhtzc35x_F1Cj2B939q04</recordid><startdate>200906</startdate><enddate>200906</enddate><creator>Matsuzawa, M.</creator><creator>Sheu, T.-J.</creator><creator>Lee, Y.-J.</creator><creator>Chen, M.</creator><creator>Li, T.-F.</creator><creator>Huang, C. T.</creator><creator>Holz, J. D.</creator><creator>Puzas, J. E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7X8</scope></search><sort><creationdate>200906</creationdate><title>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</title><author>Matsuzawa, M. ; Sheu, T.-J. ; Lee, Y.-J. ; Chen, M. ; Li, T.-F. ; Huang, C. T. ; Holz, J. D. ; Puzas, J. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Alveolar Process - metabolism</topic><topic>amelogenin</topic><topic>Amelogenin - biosynthesis</topic><topic>Amelogenin - genetics</topic><topic>Amelogenin - physiology</topic><topic>Animals</topic><topic>beta Catenin - biosynthesis</topic><topic>beta Catenin - metabolism</topic><topic>beta-Galactosidase - biosynthesis</topic><topic>Biological and medical sciences</topic><topic>bone formation</topic><topic>cell signaling</topic><topic>Cells, Cultured</topic><topic>Dentistry</topic><topic>Gene Expression</topic><topic>Genes, Reporter</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Transgenic</topic><topic>osteoblast</topic><topic>Osteoblasts - metabolism</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>periodontal ligament</topic><topic>Periodontal Ligament - cytology</topic><topic>Periodontal Ligament - metabolism</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Signal Transduction - physiology</topic><topic>TCF Transcription Factors - metabolism</topic><topic>Tooth Root - metabolism</topic><topic>Transfection</topic><topic>Wnt Proteins - metabolism</topic><topic>β-catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Matsuzawa, M.</creatorcontrib><creatorcontrib>Sheu, T.-J.</creatorcontrib><creatorcontrib>Lee, Y.-J.</creatorcontrib><creatorcontrib>Chen, M.</creatorcontrib><creatorcontrib>Li, T.-F.</creatorcontrib><creatorcontrib>Huang, C. T.</creatorcontrib><creatorcontrib>Holz, J. D.</creatorcontrib><creatorcontrib>Puzas, J. E.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium &amp; Calcified Tissue Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of periodontal research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Matsuzawa, M.</au><au>Sheu, T.-J.</au><au>Lee, Y.-J.</au><au>Chen, M.</au><au>Li, T.-F.</au><au>Huang, C. T.</au><au>Holz, J. D.</au><au>Puzas, J. E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway</atitle><jtitle>Journal of periodontal research</jtitle><addtitle>J Periodontal Res</addtitle><date>2009-06</date><risdate>2009</risdate><volume>44</volume><issue>3</issue><spage>289</spage><epage>296</epage><pages>289-296</pages><issn>0022-3484</issn><eissn>1600-0765</eissn><abstract>Background and Objective:  While it has long been known that amelogenin is essential for the proper development of enamel, its role has generally been seen as structural in nature. However, our new data implicate this protein in the regulation of cell signaling pathways in periodontal ligament cells and osteoblasts. In this article we report the successful purification of a recombinant mouse amelogenin protein and demonstrate that it has signaling activity in isolated mouse calvarial cells and human periodontal ligament cells. Material and Methods:  To determine the regulatory function of canonical Wnt signaling by amelogenin, we used TOPGAL transgenic mice. These mice express a β‐galactosidase transgene under the control of a LEF/TCF and β‐catenin‐inducible promoter. To investigate in greater detail the molecular mechanisms involved in the β‐catenin signaling pathway, isolated osteoblasts and periodontal ligament cells were exposed to full‐length recombinant mouse amelogenin and were evaluated for phenotypic changes and β‐catenin signaling using a TOPFLASH construct and the LacZ reporter gene. Results:  In these in vitro models, we showed that amelogenin can activate β‐catenin signaling. Conclusion:  Using the TOPGAL transgenic mouse we showed that amelogenin expression in vivo is localized mainly around the root, the periodontal ligament and the alveolar bone.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>19462488</pmid><doi>10.1111/j.1600-0765.2008.01091.x</doi><tpages>8</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0022-3484
ispartof Journal of periodontal research, 2009-06, Vol.44 (3), p.289-296
issn 0022-3484
1600-0765
language eng
recordid cdi_proquest_miscellaneous_67277641
source Wiley-Blackwell Read & Publish Collection
subjects Alveolar Process - metabolism
amelogenin
Amelogenin - biosynthesis
Amelogenin - genetics
Amelogenin - physiology
Animals
beta Catenin - biosynthesis
beta Catenin - metabolism
beta-Galactosidase - biosynthesis
Biological and medical sciences
bone formation
cell signaling
Cells, Cultured
Dentistry
Gene Expression
Genes, Reporter
Humans
Medical sciences
Mice
Mice, Transgenic
osteoblast
Osteoblasts - metabolism
Otorhinolaryngology. Stomatology
periodontal ligament
Periodontal Ligament - cytology
Periodontal Ligament - metabolism
Recombinant Proteins - pharmacology
Signal Transduction - physiology
TCF Transcription Factors - metabolism
Tooth Root - metabolism
Transfection
Wnt Proteins - metabolism
β-catenin
title Putative signaling action of amelogenin utilizes the Wnt/β-catenin pathway
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T13%3A55%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Putative%20signaling%20action%20of%20amelogenin%20utilizes%20the%20Wnt/%CE%B2-catenin%20pathway&rft.jtitle=Journal%20of%20periodontal%20research&rft.au=Matsuzawa,%20M.&rft.date=2009-06&rft.volume=44&rft.issue=3&rft.spage=289&rft.epage=296&rft.pages=289-296&rft.issn=0022-3484&rft.eissn=1600-0765&rft_id=info:doi/10.1111/j.1600-0765.2008.01091.x&rft_dat=%3Cproquest_cross%3E20545367%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c4661-d539e4c1451e5317490e6e3e60b3c54a5970dffdd55f26c56cf90f8687b02fe93%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=20545367&rft_id=info:pmid/19462488&rfr_iscdi=true