Effects of Moritella viscosa antigens on pro-inflammatory gene expression in an Atlantic salmon ( Salmo salar Linnaeus) cell line (SHK-1)

• Published in: Fish & shellfish immunology 2009-06, Vol.26 (6), p.858-863
• Main Authors: Bjornsdottir, Bryndis, Fast, Mark D., Sperker, Sandra A., Brown, Laura L., Gudmundsdottir, Bjarnheidur K.
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Bacterial - immunology; Atlantic salmon; Brackish; Cell Line; Cell Survival - immunology; Extracellular products; Fish Diseases - immunology; Fish Diseases - microbiology; Freshwater; Gene Expression; Immune response; Interleukin-1beta - biosynthesis; Interleukin-1beta - genetics; Interleukin-1beta - immunology; Interleukin-1β (IL-1β); Interleukin-8 (IL-8); Interleukin-8 - biosynthesis; Interleukin-8 - genetics; Interleukin-8 - immunology; Macrophages; Marine; Metalloendopeptidases - immunology; Moritella - immunology; Moritella viscosa; MvP1 vibriolysin; Pro-inflammatory cytokine; Reverse Transcriptase Polymerase Chain Reaction - veterinary; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Salmo salar; SHK-1; Vibrio Infections - immunology; Vibrio Infections - microbiology; Vibrio Infections - veterinary
• ISSN: 1050-4648; 1095-9947
• DOI: 10.1016/j.fsi.2009.03.015

Moritella viscosa is the causative agent of winter ulcer disease in salmonids reared in North-Atlantic countries. In this study the effects of selected M. viscosa antigens on cytotoxicity and pro-inflammatory gene expression in an Atlantic salmon ( Salmo salar Linnaeus) macrophage-like cell line (SHK-1) were examined. SHK-1 cells were stimulated with live and heat-killed bacterial cells, extracellular products (ECP) and an extracellular vibriolysin, termed MvP1. Following incubation, cytotoxicity and expression levels of interleukin-1β (IL-1β) and interleukin-8 (IL-8) were examined at different time points. Both live M. viscosa cells and ECP were cytotoxic, but neither heat-killed cells, nor the MvP1 peptidase caused cell death. Expression levels of both IL-1β and IL-8 increased significantly after stimulation with live cells, but heat-killed cells only caused increased IL-8 expression. ECP did not affect IL-1β expression, but did stimulate IL-8 expression. The isolated MvP1 peptidase stimulated both IL-1β and IL-8 expression at the highest concentration tested. This study reveals a difference in the induction of pro-inflammatory gene expression in salmon SHK-1 cells between live and heat-killed M. viscosa cells, and also that an unknown secreted factor is the main stimulant of IL-β and IL-8 expression.