Pathogenic bacteria and TNF do not induce production of macrophage migration inhibitory factor (MIF) by human monocytes

Elevated serum macrophage migration inhibitory factor (MIF) is associated with severe sepsis, but it is not clear whether bacteria stimulate synthesis of MIF by blood leukocytes directly or via induction of TNF. Here we assess production of MIF mRNA and protein by blood leukocytes from healthy human...

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Published in:Cytokine (Philadelphia, Pa.) Pa.), 2009-06, Vol.46 (3), p.316-318
Main Authors: Temple, Suzanna E.L., Cheong, Karey Y., Price, Patricia, Waterer, Grant W.
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
Escherichia coli
Escherichia coli - immunology
Escherichia coli - pathogenicity
Humans
Macrophage Migration-Inhibitory Factors - genetics
Macrophage Migration-Inhibitory Factors - metabolism
MIF
Monocytes
Monocytes - cytology
Monocytes - immunology
RNA - metabolism
Sepsis - immunology
Sepsis - microbiology
Streptococcus pneumoniae
Streptococcus pneumoniae - immunology
Streptococcus pneumoniae - pathogenicity
TNF
Tumor Necrosis Factor-alpha - immunology
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Summary:Elevated serum macrophage migration inhibitory factor (MIF) is associated with severe sepsis, but it is not clear whether bacteria stimulate synthesis of MIF by blood leukocytes directly or via induction of TNF. Here we assess production of MIF mRNA and protein by blood leukocytes from healthy human subjects ( n = 28) following exposure to bacteria commonly associated with sepsis ( Escherichia coli and Streptococcus pneumoniae). Bacteria did not increase levels of MIF mRNA or secreted protein. CD14 + monocytes were the main cell type producing MIF before and after stimulation. Exposure of leukocytes to TNF did not induce MIF. Hence elevated levels of serum MIF observed in sepsis may not reflect MIF produced by blood leukocytes stimulated directly by bacteria or TNF.
ISSN:1043-4666
1096-0023
DOI:10.1016/j.cyto.2009.03.008