SPARC is a Major Secretory Gene Expressed and Involved in the Development of Proliferative Diabetic Retinopathy

Aim: Neovascularization is an important event in proliferative diabetic retinopathy (PDR), where various secretory proteins including multiple growth factors are considered to be involved in this process. We searched for secretory proteins expressed in a surgical specimen obtained from the eyes of p...

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Bibliographic Details
Published in:Journal of Atherosclerosis and Thrombosis 2009, Vol.16(2), pp.69-76
Main Authors: Watanabe, Kazuhisa, Okamoto, Fumiki, Yokoo, Tomotaka, Iida, Kaoruko Tada, Suzuki, Hiroaki, Shimano, Hitoshi, Oshika, Tetsuro, Yamada, Nobuhiro, Toyoshima, Hideo
Format: Article
Language:English
Subjects:
Animals
Diabetic Retinopathy - etiology
Diabetic Retinopathy - pathology
Eye Proteins - analysis
Eye Proteins - genetics
Gene Library
Humans
Neovascularization
Osteonectin - analysis
Proliferative diabetic retinopathy
Rats
Secreted protein acidic and rich in cystein
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Summary:Aim: Neovascularization is an important event in proliferative diabetic retinopathy (PDR), where various secretory proteins including multiple growth factors are considered to be involved in this process. We searched for secretory proteins expressed in a surgical specimen obtained from the eyes of patients with PDR. Methods: We developed the oligo-cap signal sequence trap (SST) strategy which enables us to screen for secretory or membrane proteins from a minimal starting material. Using this method, we were able to screen a cDNA library constructed from a surgical specimen obtained from the eyes of the patients with PDR. Results: Majority of the cloned cDNAs turned out to encode secreted protein acidic and rich in cystein (SPARC), strongly suggesting that SPARC is highly expressed in PDR. Analysis of vitreous fluid from various patients has shown that the concentration of SPARC protein is increased in patients with PDR. Furthermore, subretinal injection of recombinant SPARC adenovirus induced PDR-like changes in the rat eye. Conclusions: Our results strongly suggested that SPARC is involved in the development of diabetic retinopathy (DR).
ISSN:1340-3478
1880-3873
DOI:10.5551/jat.E711