Association of the human bradykinin B2 receptor gene with chronic renal failure

• Published in: Molecular diagnosis 2004, Vol.8 (3), p.157-161
• Main Authors: Jozwiak, Lucyna, Drop, Andrzej, Buraczynska, Kinga, Ksiazek, Piotr, Mierzicki, Piotr, Buraczynska, Monika
• Format: Article
• Language: English
• Subjects: Adult; Exons; Female; Genetic Predisposition to Disease; Humans; Kallikrein-Kinin System - genetics; Kidney Failure, Chronic - genetics; Male; Polymorphism, Genetic; Receptor, Bradykinin B2 - genetics
• ISSN: 1084-8592
• DOI: 10.1007/BF03260059

The kallikrein-kinin system plays an important role in blood pressure homeostasis and renal sodium regulation, and some studies have reported that the kinins have a protective effect against hypertension and the development of renal disease. The B2-bradykinin receptor (B2R) mediates the majority of physiological actions of bradykinin. We investigated the effect of the C181-->T polymorphism in exon 2 of the B2R gene in patients with end-stage renal disease (ESRD). This study involved 790 patients with ESRD and 510 healthy controls. All participants were genotyped for the B2R C181-->T polymorphism by PCR followed by digestion of a PCR product with TaqI restriction endonuclease. DNA fragments were separated by agarose gel electrophoresis. Genotype and allele frequencies were compared between the groups. All calculations were performed using SPSS 5.0 for Windows. B2R genotype distribution in patients and controls was in accordance with Hardy-Weinberg equilibrium. The frequency of the T allele was higher in ESRD patients than in controls. The significant difference was observed in the age at onset of renal disease; for patients with the T allele the mean age at onset was 36.8 years, compared with 52.4 years for those carrying only the C allele (p