Relationship between semen characteristics and body size in Barbus barbus L. (Teleostei: Cyprinidae) and effects of ions and osmolality on sperm motility

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2009-08, Vol.153 (4), p.430-437
• Main Authors: Alavi, S.M. Hadi, Rodina, Marek, Policar, Tomáš, Linhart, Otomar
• Format: Article
• Language: English
• Subjects: Animals; Barbus barbus; Body Size; Cyprinidae; Flagellum; Freshwater; Ions; Male; Motility; Osmolar Concentration; Semen; Solutions; Sperm Motility; Sperm trajectory; Sperm volume; Spermatozoa concentration; Total number of spermatozoa; Velocity
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2009.04.001

The objectives of the present study were to determine the relationships among length and weight of males, sperm volume, spermatozoa concentration, total number of spermatozoa, ionic contents and osmolality of seminal plasma in Barbus barbus. The effect of osmolality on sperm motility parameters after activation in NaCl, KCl, or sucrose solutions was also examined. There were significant correlations between spermatozoa concentration – length ( R = + 0.7) and – weight ( R = + 0.8) of males. No significant correlations were observed between the total number of spermatozoa, sperm volume, and length and weight of males. Seminal plasma osmolality was higher when the total number of spermatozoa ( R = + 0.6) and sperm volume ( R = + 0.6) were higher. Sperm motility and velocity was positively correlated with osmolality ( R = + 0.5). The correlation between sperm motility and K + was negative ( R = 0.5), but positively correlated with Ca 2+ ( R = 0.8), Na + ( R = 0.8), and Cl − ( R = 0.8). There was a rapid decrease ( P < 0.05) in sperm motility parameters after sperm activation. Just after sperm activation, beating waves propagated along the full length of flagella. At latter stages post sperm activation, the waves appeared only in proximal part of the flagellum. The highest spermatozoa velocity and percentage of motility were observed at 215–235 mOsmol kg − 1 in NaCl, KCl or sucrose. The tip of the flagellum became curled into a loop shape which shortened the flagellum after activation of sperm in distilled water. B. barbus sperm is very similar to that of other cyprinids in terms of ionic contents and osmolality of the seminal plasma, mechanism of sperm activation and behavior and motility of sperm during swimming period.