Campylobacter Colonization of the Turkey Intestine in the Context of Microbial Community Development

Patterns of microbial community dynamics in the turkey intestine were examined. Every week of the 18-week production cycle, cecal bacterial communities and Campylobacter loads were examined from five birds for each of two flocks. Molecular fingerprinting via the automated ribosomal intergenic spacer...

Full description

Saved in:
Bibliographic Details
Published in:Applied and Environmental Microbiology 2009-06, Vol.75 (11), p.3564-3571
Main Author: Scupham, Alexandra J
Format: Article
Language:English
Subjects:
Animals
automated ribosomal intergenic spacer analysis
Bacteria
Bacteria - classification
Bacteria - isolation & purification
bacterial colonization
Bacteriology
Biological and medical sciences
Campylobacter
Campylobacter - growth & development
Campylobacter coli
Cecum
Cecum - microbiology
Cluster Analysis
Colony Count, Microbial
Digestive system
DNA Fingerprinting
DNA, Bacterial - genetics
DNA, Ribosomal Spacer - genetics
Food Microbiology
Fundamental and applied biological sciences. Psychology
intestines
Microbiology
Polymorphism
Polymorphism, Genetic
restriction fragment length polymorphism
Ribonucleic acid
RNA
terminal restriction fragment length polymorphism
turkeys
Turkeys - microbiology
Wildfowl
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Patterns of microbial community dynamics in the turkey intestine were examined. Every week of the 18-week production cycle, cecal bacterial communities and Campylobacter loads were examined from five birds for each of two flocks. Molecular fingerprinting via the automated ribosomal intergenic spacer analysis (ARISA) and terminal restriction fragment length polymorphism (T-RFLP) of the cecal samples revealed that microbial communities changed in a time-dependent manner, and during both trials they developed via transition through three phases during the production cycle. A core component of the microbiota consisting of 11 Bacteroidetes types was present throughout both trials. In contrast, constant succession was detected in the Clostridiales populations until week 10 or 11, with few shared sequences between the flocks. Changes in Campylobacter jejuni and Campylobacter coli loads were correlated to, but not dependent on, the two acute transitions delimiting the three developmental phases.
ISSN:0099-2240
1098-5336
1098-6596
DOI:10.1128/AEM.01409-08