Isolation and characterization of an alkaliphilic bacterium utilizing pyrene as a carbon source

Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic aci...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2004, Vol.98 (4), p.306-308
Main Authors: Habe, Hiroshi, Kanemitsu, Mieko, Nomura, Mizuki, Takemura, Tetsuo, Iwata, Kenichi, Nojiri, Hideaki, Yamane, Hisakazu, Omori, Toshio
Format: Article
Language:English
Subjects:
ALKALINIZATION
alkaliphile
Biological and medical sciences
BIOREMEDIATION
Biotechnology
CARBON
CHEMICALS
Fundamental and applied biological sciences. Psychology
MYCOBACTERIUM
Mycobacterium sp
polycyclic aromatic hydrocarbons
pyrene degradation
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Summary:Alkaliphilic Mycobacterium sp. strain MHP-1, which can grow on pyrene as a sole carbon and energy source, was isolated from a soil sample. At the optimum pH for growth (pH 9), about 50% of pyrene (final concentration at 0.1% [w/v]) was degraded during 7 d of incubation, and 4,5-phenanthrenedioic acid, 4-phenanthroic acid and phthalic acid were identified as metabolic intermediates. Strain MHP-1 was found to possess aromatic-ring dioxygenase genes, which are highly homologous to the known nidAB genes from pyrene-degrading mycobacteria.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(04)00287-7