Cloning and expression of a NAD +-dependent xylitol dehydrogenase gene ( xdhA) of Aspergillus oryzae

XdhA, which encodes a xylitol dehydrogenase gene, was cloned from Aspergillus oryzae genomic DNA. It consists of 1214 bp structural region, which is interrupted by two introns, and encodes 358-amino-acid protein (38,197 Da). It is similar to the known NAD +-dependent xylitol dehydrogenase (EC 1.1.1....

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Bibliographic Details
Published in:Journal of Bioscience and Bioengineering 2004, Vol.97 (6), p.419-422
Main Authors: Tran, Lien Ha, Kitamoto, Noriyuki, Kawai, Keiichi, Takamizawa, Kazuhiro, Suzuki, Tohru
Format: Article
Language:English
Subjects:
ASPERGILLUS ORYZAE
Biological and medical sciences
Biotechnology
D-xylulose reductase
expressed sequence tag (EST)
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
genomic DNA
MOLECULAR CLONING
oxidoreductase
OXIDOREDUCTASES
XYLITOL
xylitol dehydrogenase
xylose metabolism
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Summary:XdhA, which encodes a xylitol dehydrogenase gene, was cloned from Aspergillus oryzae genomic DNA. It consists of 1214 bp structural region, which is interrupted by two introns, and encodes 358-amino-acid protein (38,197 Da). It is similar to the known NAD +-dependent xylitol dehydrogenase (EC 1.1.1.9). The gene was expressed in Escherichia coli BL21-AI using a T7 promoter. The cell-free extract of the transformant showed a 36.5 kDa band upon SDS–PAGE and NAD +-dependent xylitol dehydrogenase activity.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(04)70229-7