Improvement of bacterial cellulose production by addition of agar in a jar fermentor

Bacterial cellulose (BC) was produced by Acetobacter xylinum BPR 2001 and its acetan nonproducing mutant EP1 in corn steep liquor-fructose medium in a 10- l jar fermentor supplemented with different agar concentrations ranging from 0% to 1.0% (w/v). The BC productivity of the two strains was increas...

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Bibliographic Details
Published in:Journal of Bioscience and Bioengineering 2004, Vol.97 (1), p.33-38
Main Authors: Bae, Sangok, Sugano, Yasushi, Shoda, Makoto
Format: Article
Language:English
Subjects:
acetan
ACETOBACTER
Acetobacter xylinum
AGAR
bacterial cellulose
Biological and medical sciences
Biotechnology
CELLULOSE
FERMENTERS
Fundamental and applied biological sciences. Psychology
jar fermentor
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Summary:Bacterial cellulose (BC) was produced by Acetobacter xylinum BPR 2001 and its acetan nonproducing mutant EP1 in corn steep liquor-fructose medium in a 10- l jar fermentor supplemented with different agar concentrations ranging from 0% to 1.0% (w/v). The BC productivity of the two strains was increased by adding agar. The maximum BC production of BPR 2001 at an agar concentration of 0.4% was 12.8 g/ l compared with 8 g/ l without agar. The mutant EP1 produced 11.6 g/ l of BC at an agar concentration of 0.6%, while only 5.5 g/ l was produced in the control. Enhanced productivity is associated with an increase in viscosity of the culture, dispersion of BC pellets, and number of free cells due to agar addition, suggesting that acetan produced by BPR 2001 has a critical role in enhanced BC production.
ISSN:1389-1723
1347-4421
DOI:10.1016/S1389-1723(04)70162-0