Continuous nisin production with bioengineered Lactococcus lactis strains

Nisin production in continuous cultures of bioengineered Lactococcus lactis strains that incorporate additional immunity and regulation genes was studied. Highest nisin activities were observed at 0.2 h⁻¹ dilution rate and 12.5 g l⁻¹ fructose concentration for all strains. Recombinant strains were a...

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Bibliographic Details
Published in:Journal of industrial microbiology & biotechnology 2009-06, Vol.36 (6), p.863-871
Main Authors: Şimşek, Ö, Akkoç, N, Çon, A. H, Özçelik, F, Saris, P. E. J, Akçelik, Mustafa
Format: Article
Language:English
Subjects:
Biochemistry
Bioengineering
Bioinformatics
Biological and medical sciences
Biomass
Biomedical and Life Sciences
Biotechnology
Culture Media - metabolism
Engineering
FDA approval
Federal regulation
Fermentation
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Inorganic Chemistry
Kinetics
Lactococcus lactis
Lactococcus lactis - chemistry
Lactococcus lactis - genetics
Lactococcus lactis - metabolism
Life Sciences
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
Microbiology
Nisin - chemistry
Nisin - metabolism
Original Paper
Plasmids
Productivity
Studies
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Summary:Nisin production in continuous cultures of bioengineered Lactococcus lactis strains that incorporate additional immunity and regulation genes was studied. Highest nisin activities were observed at 0.2 h⁻¹ dilution rate and 12.5 g l⁻¹ fructose concentration for all strains. Recombinant strains were able to produce greater amounts of nisin at dilution rates below 0.3 h⁻¹ compared to the control strain. However, this significant difference disappeared at dilution rates of 0.4 and 0.5 h⁻¹. For the strains LL27, LAC338, LAC339, and LAC340, optimum conditions for nisin production were determined to be at 0.29, 0.26, 0.27, and 0.27 h⁻¹ dilution rates and 11.95, 12.01, 11.63, and 12.50 g l⁻¹ fructose concentrations, respectively. The highest nisin productivity, 496 IU ml⁻¹ h⁻¹, was achieved with LAC339. The results of this study suggest that low dilution rates stabilize the high specific nisin productivity of the bioengineered strains in continuous fermentation. Moreover, response surface methodology analysis showed that regulation genes yielded high nisin productivity at wide ranges of dilution rates and fructose concentrations.
ISSN:1367-5435
1476-5535
DOI:10.1007/s10295-009-0563-6