Depigmentation of Melanocytes by (2Z,8Z)-Matricaria Acid Methyl Ester Isolated from Erigeron breviscapus

To discover an active skin depigmenting agent, we isolated a novel inhibitor of melanin biosynthesis from the methanol extract of Erigeron breviscapus using a bioactivity-guided fractionation and identified it as (2Z,8Z)-matricaria acid methyl ester by means of spectroscopic analysis. The compound s...

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Bibliographic Details
Published in:Biological & Pharmaceutical Bulletin 2009/06/01, Vol.32(6), pp.1091-1094
Main Authors: Luo, Lian Hua, Kim, Hae Jong, Nguyen, Dung Hoang, Lee, Hyang-Bok, Lee, Nam Ho, Kim, Eun-Ki
Format: Article
Language:English
Subjects:
(2Z,8Z)-matricaria acid methyl ester
Animals
Blotting, Western
Cell Line, Tumor
Drugs, Chinese Herbal - isolation & purification
Drugs, Chinese Herbal - pharmacology
Erigeron - chemistry
Erigeron breviscapus
melanin
Melanins - antagonists & inhibitors
Melanins - biosynthesis
Melanocytes - drug effects
Melanocytes - enzymology
Melanocytes - metabolism
Mice
Molecular Structure
Monophenol Monooxygenase - antagonists & inhibitors
Polyynes - isolation & purification
Polyynes - pharmacology
skin whitening
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Summary:To discover an active skin depigmenting agent, we isolated a novel inhibitor of melanin biosynthesis from the methanol extract of Erigeron breviscapus using a bioactivity-guided fractionation and identified it as (2Z,8Z)-matricaria acid methyl ester by means of spectroscopic analysis. The compound showed strong whitening activity in melan-a cell. Compared with arbutin (IC50=4.0 mM) as a positive control, the depigmentation IC50 value for (2Z,8Z)-matricaria acid methyl ester was 25.4 μM in B16F10 melanoma cell. Moreover, its inhibitory effect on tyrosinase, the key enzyme of melanogenesis, was examined by in vivo and in vitro tyrosinase assay and Western blot. The results indicate that (2Z,8Z)-matricaria acid methyl ester isolated from Erigeron breviscapus is a promising compound that could be useful for treating hyper-pigmentation as skin-whitening agents.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.32.1091