Effect of Surface Composition on the Adsorption of Photosystem I onto Alkanethiolate Self-Assembled Monolayers on Gold

We have used self-assembled monolayers (SAMs) prepared from ω-terminated alkanethiols on gold to generate model surfaces and examine the effect of surface composition on the adsorption of Photosystem I (PSI), stabilized in aqueous solution by Triton X-100. Triton-stabilized PSI adsorbs to high-energ...

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Bibliographic Details
Published in:Langmuir 2004-05, Vol.20 (10), p.4033-4038
Main Authors: Ko, B. Samuel, Babcock, Brian, Jennings, G. Kane, Tilden, S. Grey, Peterson, Rachel R, Cliffel, David, Greenbaum, Elias
Format: Article
Language:English
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Summary:We have used self-assembled monolayers (SAMs) prepared from ω-terminated alkanethiols on gold to generate model surfaces and examine the effect of surface composition on the adsorption of Photosystem I (PSI), stabilized in aqueous solution by Triton X-100. Triton-stabilized PSI adsorbs to high-energy surfaces prepared from HO- and HO2C-terminated alkanethiols but does not adsorb to low-energy surfaces. The inhibition of PSI adsorption at low-energy surfaces is consistent with the presence of a layer of Triton X-100 that adsorbs atop the hydrophobic SAM and presents a protein-resistant poly(ethylene glycol) (PEG) surface. While the presence of the PEG surface prevents the adsorption of PSI, the displacement of the inhibiting layer of Triton X-100 by dodecanol, a more active surfactant, greatly enhances the adsorption of PSI. This inhibiting effect by Triton X-100 can be extended to other protein systems such as bovine serum albumin.
ISSN:0743-7463
1520-5827
DOI:10.1021/la0356809