Evaluation of Epstein–Barr virus antibodies, anti-VCA avidity by immunofluorescence and immunoblot assays for assessment of Epstein–Barr virus immunologic state

Three hundred twenty two serum samples were evaluated by indirect immunofluorescence assay (IFA) and immunoblotting for the assessment of the immunologic status of Epstein–Barr virus (EBV). Serological profiles of 322 serum samples were classified by the IgM and IgG antibodies by the immunofluoresce...

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Bibliographic Details
Published in:Journal of virological methods 2009-08, Vol.159 (2), p.300-302
Main Authors: Sener, Asli Gamze, Afsar, Ilhan, Pinar, Ercan
Format: Article
Language:English
Subjects:
Adolescent
Adult
Age Factors
Aged
Aged, 80 and over
Antibodies, Viral - blood
Antibody
Antibody Affinity
Antigens, Viral - immunology
Avidity
Biological and medical sciences
Capsid Proteins - immunology
Child
Child, Preschool
Epstein-Barr virus
Epstein-Barr Virus Infections - immunology
Fluorescent Antibody Technique
Fundamental and applied biological sciences. Psychology
Herpesvirus 4, Human - immunology
Humans
Immunoblot
Immunoblotting
Immunoglobulin G - blood
Immunoglobulin M - blood
Microbiology
Middle Aged
Seroepidemiologic Studies
Techniques used in virology
Virology
Young Adult
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Summary:Three hundred twenty two serum samples were evaluated by indirect immunofluorescence assay (IFA) and immunoblotting for the assessment of the immunologic status of Epstein–Barr virus (EBV). Serological profiles of 322 serum samples were classified by the IgM and IgG antibodies by the immunofluorescence assay as seronegative, acute infection, reactivation and past infection. Higher rates of seropositivity were determined in serum samples belonging to old age groups. Routine tests used for EBV diagnosis were efficient.
ISSN:0166-0934
1879-0984
DOI:10.1016/j.jviromet.2009.04.009