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HPLC method validation studies on a specific assay for monomethoxypoly(ethylene glycol) succinimido carbonate (mPEG-SC)
An UV-HPLC method for the determination of the potency of mPEG-SC, 5 kDa ( 1) has been developed and validated. Validation acceptance criteria that is typical of small molecule method validation was successfully applied for this method. The method relies on the HPLC separation of mPEG-OH, 5 kDa ( 2)...
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Published in: | Journal of pharmaceutical and biomedical analysis 2009-09, Vol.50 (2), p.138-143 |
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Main Authors: | , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | An UV-HPLC method for the determination of the potency of mPEG-SC, 5
kDa (
1) has been developed and validated. Validation acceptance criteria that is typical of small molecule method validation was successfully applied for this method. The method relies on the HPLC separation of mPEG-OH, 5
kDa (
2) and a derivatized form of mPEG-SC, 5
kDa (
1). 1-Naphthylmethylamine reacts with mPEG-SC, 5
kDa (
1) to form a stable, strongly UV absorbing carbamate
3. When the eluent is analyzed by UV detection at 220
nm, the method was shown to be linear, precise and accurate. The limit of detection of the method was 0.1
μg. |
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ISSN: | 0731-7085 1873-264X |
DOI: | 10.1016/j.jpba.2009.04.005 |