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HPLC method validation studies on a specific assay for monomethoxypoly(ethylene glycol) succinimido carbonate (mPEG-SC)

An UV-HPLC method for the determination of the potency of mPEG-SC, 5 kDa ( 1) has been developed and validated. Validation acceptance criteria that is typical of small molecule method validation was successfully applied for this method. The method relies on the HPLC separation of mPEG-OH, 5 kDa ( 2)...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2009-09, Vol.50 (2), p.138-143
Main Authors: Van Arnum, Susan D., Niemczyk, Henry J., Chang, Chinfong
Format: Article
Language:English
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Summary:An UV-HPLC method for the determination of the potency of mPEG-SC, 5 kDa ( 1) has been developed and validated. Validation acceptance criteria that is typical of small molecule method validation was successfully applied for this method. The method relies on the HPLC separation of mPEG-OH, 5 kDa ( 2) and a derivatized form of mPEG-SC, 5 kDa ( 1). 1-Naphthylmethylamine reacts with mPEG-SC, 5 kDa ( 1) to form a stable, strongly UV absorbing carbamate 3. When the eluent is analyzed by UV detection at 220 nm, the method was shown to be linear, precise and accurate. The limit of detection of the method was 0.1 μg.
ISSN:0731-7085
1873-264X
DOI:10.1016/j.jpba.2009.04.005