Overexpression and Interactions of Interleukin-10, Transforming Growth Factor β, and Vascular Endothelial Growth Factor in Esophageal Squamous Cell Carcinoma

• Published in: World journal of surgery 2009-07, Vol.33 (7), p.1439-n/a
• Main Authors: Gholamin, Mehran, Moaven, Omeed, Memar, Bahram, Farshchian, Moein, Naseh, Hossein, Malekzadeh, Reza, Sotoudeh, Masoud, Rajabi-Mashhadi, Mohammad Taghi, Forghani, Mohammad Naser, Farrokhi, Farid, Abbaszadegan, Mohammad Reza
• Format: Article
• Language: English
• Subjects: Abdominal Surgery; Adult; Aged; Biological and medical sciences; Biomarkers, Tumor - metabolism; Biopsy, Needle; Carcinoma, Squamous Cell - metabolism; Carcinoma, Squamous Cell - mortality; Carcinoma, Squamous Cell - pathology; Cardiac Surgery; Chi-Square Distribution; Cohort Studies; Esophageal Cancer; Esophageal Neoplasms - metabolism; Esophageal Neoplasms - mortality; Esophageal Neoplasms - pathology; Esophageal Squamous Cell Carcinoma; Esophageal Squamous Cell Carcinoma Patient; Esophagus; Female; Gastroenterology. Liver. Pancreas. Abdomen; General aspects; General Surgery; Humans; Immunohistochemistry; Interleukin-10 - genetics; Interleukin-10 - metabolism; Major Histocompatibility Complex Class; Male; Medical sciences; Medicine; Medicine & Public Health; Middle Aged; Probability; Prognosis; Reverse Transcriptase Polymerase Chain Reaction; Risk Assessment; RNA, Messenger - analysis; Sensitivity and Specificity; Statistics, Nonparametric; Surgery; Survival Analysis; Thoracic Surgery; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - metabolism; Tumors; Vascular Endothelial Growth Factor; Vascular Endothelial Growth Factor A - genetics; Vascular Endothelial Growth Factor A - metabolism; Vascular Surgery
• ISSN: 0364-2313; 1432-2323
• DOI: 10.1007/s00268-009-0070-y

Background Sharing the role of immune suppression, interleukin-10 (IL-10), transforming growth factor β (TGF-β), and vascular endothelial growth factor (VEGF) are critical genes in several aspects of tumorigenesis. To elucidate the role of these cytokines in esophageal squamous cell carcinoma (ESCC), their relative mRNA expression in tumoral tissue compared with corresponding tumor-free tissue was evaluated. Methods A total of 49 patients with histologically confirmed ESCC were included in the study prior to any therapeutic interventions. Quantitative analysis of the mRNA expression was performed by real-time reverse transcription-polymerase chain reaction and the clinicopathologic associations were assessed. Results The mRNA of IL-10, VEGF, and TGF-β was frequently overexpressed in 53.2%, 44.9%, and 37.5% of ESCC patients, respectively. TGF-β was significantly co-expressed with IL-10 and with VEGF. Although VEGF was not independently associated with increased tumor size ( p  = 0.065), concomitant overexpression of VEGF with TGF-β was significantly correlated with increased size of the tumor ( p