Integrated microelectrode array and microfluidics for temperature clamp of sensory neurons in culture

A device for cell culture is presented that combines MEMS technology and liquid-phase photolithography to create a microfluidic chip that influences and records electrical cellular activity. A photopolymer channel network is formed on top of a multichannel microelectrode array. Preliminary results i...

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Bibliographic Details
Published in:Lab on a chip 2005-01, Vol.5 (1), p.97-101
Main Authors: Pearce, Thomas M, Wilson, J Adam, Oakes, S George, Chiu, Shing-Yan, Williams, Justin C
Format: Article
Language:English
Subjects:
Action Potentials
Animals
Equipment Design
Ganglia, Spinal - cytology
Ganglia, Spinal - physiology
Microelectrodes
Microfluidic Analytical Techniques - instrumentation
Microfluidic Analytical Techniques - methods
Neurons, Afferent - cytology
Neurons, Afferent - physiology
Patch-Clamp Techniques
Temperature
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Summary:A device for cell culture is presented that combines MEMS technology and liquid-phase photolithography to create a microfluidic chip that influences and records electrical cellular activity. A photopolymer channel network is formed on top of a multichannel microelectrode array. Preliminary results indicated successful local thermal control within microfluidic channels and control of lamina position over the electrode array. To demonstrate the biological application of such a device, adult dissociated dorsal root ganglion neurons with a subpopulation of thermally-sensitive cells are attached onto the electrode array. Using laminar flow, dynamic control of local temperature of the neural cells was achieved while maintaining a constant chemical culture medium. Recording the expected altered cellular activity confirms the success of the integrated device.
ISSN:1473-0197
1473-0189
DOI:10.1039/b407871c