Macrophages and Mast Cells Control the Neutrophil Migration Induced by Dentin Proteins

Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), the major dentin proteins, have been shown to induce neutrophil migration through release of IL-1β, TNF-α, MIP-2, and KC. However, the sources of these mediators were not determined. Here, the roles of macrophages and mast cells (MC) in dent...

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Bibliographic Details
Published in:Journal of dental research 2005-01, Vol.84 (1), p.79-83
Main Authors: Silva, T.A., Lara, V.S., Silva, J.S., Oliveira, S.H.P., Butler, W.T., Cunha, F.Q.
Format: Article
Language:English
Subjects:
Animals
Anti-Inflammatory Agents - pharmacology
Chemokine CXCL2
Chemokines - antagonists & inhibitors
Chemokines - metabolism
Chemotaxis, Leukocyte
Dentin - chemistry
Dentin - physiology
Dentistry
Dexamethasone - pharmacology
Extracellular Matrix Proteins
Interleukin-1 - antagonists & inhibitors
Interleukin-1 - metabolism
Interleukin-8 - metabolism
Macrophages - physiology
Male
Mast Cells - physiology
Mice
Mice, Inbred BALB C
Neutrophils - physiology
Phosphoproteins - physiology
Protein Precursors
Rats
Sialoglycoproteins - physiology
Tumor Necrosis Factor-alpha - antagonists & inhibitors
Tumor Necrosis Factor-alpha - metabolism
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Summary:Dentin sialoprotein (DSP) and dentin phosphoprotein (DPP), the major dentin proteins, have been shown to induce neutrophil migration through release of IL-1β, TNF-α, MIP-2, and KC. However, the sources of these mediators were not determined. Here, the roles of macrophages and mast cells (MC) in dentin-induced neutrophil accumulation were investigated. Peritoneal MC depletion or the enhancement of macrophage population increased DSP- and DPP-induced neutrophil extravasation. Moreover, supernatants from DSP- and DPP-stimulated macrophages caused neutrophil migration. The release of neutrophil chemotactic factor by macrophages was inhibited by dexamethasone or the supernatant of DSP-treated MC. Consistently, dexamethasone and the MC supernatant inhibited the production of IL-1β, TNF-α, and MIP-2 by macrophages. This inhibitory activity of the DSP-stimulated MC was neutralized by anti-IL-4 and anti-IL-10 antibodies. These results indicate that dentin induces the release of the neutrophil chemotactic substance(s) by macrophages, which are down-modulated by MC-derived IL-4 and IL-10.
ISSN:0022-0345
1544-0591
DOI:10.1177/154405910508400114