OXA-58, a novel class D {beta}-lactamase involved in resistance to carbapenems in Acinetobacter baumannii

A carbapenem-resistant Acinetobacter baumannii strain was isolated in Toulouse, France, in 2003. Cloning and expression in Escherichia coli identified the carbapenem-hydrolyzing beta-lactamase OXA-58, which is weakly related (less than 50% amino acid identity) to other oxacillinases. It hydrolyzed p...

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Bibliographic Details
Published in:Antimicrobial agents and chemotherapy 2005-01, Vol.49 (1), p.202-208
Main Authors: Poirel, Laurent, Marqué, Sophie, Héritier, Claire, Segonds, Christine, Chabanon, Gérard, Nordmann, Patrice
Format: Article
Language:English
Subjects:
Acinetobacter baumannii - drug effects
Acinetobacter baumannii - enzymology
Amino Acid Sequence
Anti-Bacterial Agents - pharmacology
Base Sequence
beta-Lactam Resistance
beta-Lactamases - chemistry
beta-Lactamases - classification
beta-Lactamases - genetics
beta-Lactamases - metabolism
Carbapenems - pharmacology
Humans
Microbial Sensitivity Tests
Molecular Sequence Data
Sequence Analysis, DNA
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Summary:A carbapenem-resistant Acinetobacter baumannii strain was isolated in Toulouse, France, in 2003. Cloning and expression in Escherichia coli identified the carbapenem-hydrolyzing beta-lactamase OXA-58, which is weakly related (less than 50% amino acid identity) to other oxacillinases. It hydrolyzed penicillins, oxacillin, and imipenem but not expanded-spectrum cephalosporins. The bla(OXA-58) gene was located on a ca. 30-kb non-self-transferable plasmid. After electrotransformation in the A. baumannii CIP7010(T) reference strain, it conferred reduced susceptibility to carbapenems. The bla(OXA-58) gene was bracketed by two novel ISAba3-like insertion elements. This study describes a newly characterized beta-lactamase that may contribute to carbapenem resistance in A. baumannii.
ISSN:0066-4804