Alternative promotion of the mouse acyl-CoA synthetase 6 (mAcsl6) gene mediates the expression of multiple transcripts with 5′-end heterogeneity: genetic organization of mAcsl6 variants

• Published in: Biochemical and biophysical research communications 2005-02, Vol.327 (1), p.84-93
• Main Authors: Lee, Eun Ju, Kim, Hi Chul, Cho, Yong Yeon, Byun, Sung June, Lim, Jeong Mook, Ryoo, Zae Young
• Format: Article
• Language: English
• Subjects: 5' Untranslated Regions - genetics; 5' Untranslated Regions - metabolism; Alternative promoter; Alternative splicing; Alternative Splicing - genetics; Amino Acid Sequence; Animals; Base Sequence; Cell Line, Tumor; Coenzyme A Ligases - chemistry; Coenzyme A Ligases - classification; Coenzyme A Ligases - genetics; Coenzyme A Ligases - metabolism; Exons - genetics; Gene Expression Regulation, Enzymologic; Genetic Variation - genetics; id vector; Introns - genetics; mAcsl6 variants; Mice; Molecular Sequence Data; Organization; Promoter Regions, Genetic - genetics; Sequence Alignment; Transcription Initiation Site; Transcription, Genetic - genetics
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2004.11.141

We report four variants and alternative promoter usage for the mouse acyl-CoA synthetase 6 (mAcsl6) gene. The variants, which were organized into 26 exons and 25 introns spanning 55 kb of DNA on mouse chromosome 11, were classified according to their 5′-UTRs and alternative splicing of exon 13. Alignment of the nucleotide sequences showed that the mAcsl6 variant 1 (mAcsl6_v1) and mAcsl6_v2 used a different promoter and had different splicing patterns than mAcsl6_v3 and mAcsl6_v4. The results of the promoter analysis suggest that the mAcsl6 promoter 1 (mAcsl6_pr1) region has a negative regulatory function. To verify this result, we constructed id vector constructs that contained the promoter regions mAcsl6_pr1 and 2, and the chimeric transcript. Although the mAcsl6_pr1 region was deleted, the mAcsl6_v1 and 2 transcripts were detected consistently.