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Crystal structure of human PNP complexed with hypoxanthine and sulfate ion
Purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme, which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. Human PNP has been submitted to intensive structure-based d...
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| Published in: | Biochemical and biophysical research communications 2005-01, Vol.326 (2), p.335-338 |
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| Main Authors: | , , , , , , |
| Format: | Article |
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| cited_by | cdi_FETCH-LOGICAL-c383t-856540e75b307e54c7cdea77cb9756c3cc0c9b638172667cf24c0ab5d1c738463 |
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| cites | cdi_FETCH-LOGICAL-c383t-856540e75b307e54c7cdea77cb9756c3cc0c9b638172667cf24c0ab5d1c738463 |
| container_end_page | 338 |
| container_issue | 2 |
| container_start_page | 335 |
| container_title | Biochemical and biophysical research communications |
| container_volume | 326 |
| creator | Canduri, Fernanda Fadel, Valmir Dias, Marcio Vinícius Bertacine Basso, Luiz Augusto Palma, Mário Sérgio Santos, Diógenes Santiago de Azevedo, Walter Filgueira |
| description | Purine nucleoside phosphorylase (PNP) is a ubiquitous enzyme, which plays a key role in the purine salvage pathway, and PNP deficiency in humans leads to an impairment of T-cell function, usually with no apparent effects on B-cell function. Human PNP has been submitted to intensive structure-based design of inhibitors, most of them using low-resolution structures of human PNP. Here we report the crystal structure of human PNP in complex with hypoxanthine, refined to 2.6
Å resolution. The intermolecular interaction between ligand and PNP is discussed. |
| doi_str_mv | 10.1016/j.bbrc.2004.11.038 |
| format | article |
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Å resolution. The intermolecular interaction between ligand and PNP is discussed.</description><subject>Crystallography, X-Ray</subject><subject>Drug design</subject><subject>Humans</subject><subject>Hypoxanthine</subject><subject>Hypoxanthine - chemistry</subject><subject>Hypoxanthine - metabolism</subject><subject>Ions - chemistry</subject><subject>Ions - metabolism</subject><subject>Ligands</subject><subject>Models, Molecular</subject><subject>Molecular Structure</subject><subject>PNP</subject><subject>Protein Conformation</subject><subject>Purine-Nucleoside Phosphorylase - chemistry</subject><subject>Purine-Nucleoside Phosphorylase - metabolism</subject><subject>Structure</subject><subject>Sulfates - chemistry</subject><subject>Sulfates - metabolism</subject><subject>Synchrotron radiation</subject><issn>0006-291X</issn><issn>1090-2104</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r3DAURUVoaSZp_0AWRavu7DxJtmRDN2VI2oTQZtFCd0J-fmY0-GMqyUnm39fDDHTXwIO7Ofc-OIxdCcgFCH29zZsmYC4BilyIHFR1xlYCasikgOINWwGAzmQtfp-zixi3AEIUun7HzkVZVnK5Fbtfh31MrucxhRnTHIhPHd_Mgxv54_dHjtOw6-mFWv7s04Zv9rvpxY1p40fibmx5nPvOJeJ-Gt-zt53rI3045SX7dXvzc_0te_jx9W795SFDVamUVaUuCyBTNgoMlQUabMkZg01tSo0KEbButKqEkVob7GSB4JqyFWhUVWh1yT4dd3dh-jNTTHbwEanv3UjTHK02SlWylq-CEpYftSkWUB5BDFOMgTq7C35wYW8F2INqu7UH1fag2gphF9VL6eNpfW4Gav9VTm4X4PMRoEXGk6dgI3oakVofCJNtJ_-__b8shI8k</recordid><startdate>20050114</startdate><enddate>20050114</enddate><creator>Canduri, Fernanda</creator><creator>Fadel, Valmir</creator><creator>Dias, Marcio Vinícius Bertacine</creator><creator>Basso, Luiz Augusto</creator><creator>Palma, Mário Sérgio</creator><creator>Santos, Diógenes Santiago</creator><creator>de Azevedo, Walter Filgueira</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050114</creationdate><title>Crystal structure of human PNP complexed with hypoxanthine and sulfate ion</title><author>Canduri, Fernanda ; 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| ispartof | Biochemical and biophysical research communications, 2005-01, Vol.326 (2), p.335-338 |
| issn | 0006-291X 1090-2104 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Crystallography, X-Ray Drug design Humans Hypoxanthine Hypoxanthine - chemistry Hypoxanthine - metabolism Ions - chemistry Ions - metabolism Ligands Models, Molecular Molecular Structure PNP Protein Conformation Purine-Nucleoside Phosphorylase - chemistry Purine-Nucleoside Phosphorylase - metabolism Structure Sulfates - chemistry Sulfates - metabolism Synchrotron radiation |
| title | Crystal structure of human PNP complexed with hypoxanthine and sulfate ion |
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